LOCUS       BBa_J70308              2657 bp    DNA     linear       25-Nov-2024
DEFINITION  R0010.E0021.J70307.E0031
ACCESSION   BBa_J70308
VERSION     BBa_J70308.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..227
                     /label=Clontech RBS
     CDS             228..944
                     /label=ECFP
     misc_feature    945..947
                     /label=stop
     misc_feature    209..948
                     /label=BBa_E0021
     misc_feature    957..959
                     /label=PsrI 
     misc_feature    1013..1020
                     /label=scar
     misc_feature    966..968
                     /label=PsrI
     misc_feature    969..1012
                     /label=B0012
     misc_feature    1021..1075
                     /label=R0011
     misc_feature    1076..1083
                     /label=scar
     misc_feature    1084..1815
                     /label=J06504 mCherry 
     misc_feature    1816..1823
                     /label=scar
     misc_feature    1824..1906
                     /label=B0012
     misc_feature    1893..1906
                     /label=PsrI
     misc_feature    957..1909
                     /label=BBa_J70307
     RBS             1919..1937
                     /label=Clontech RBS
     CDS             1938..2653
                     /label=EYFP
     misc_feature    2654..2656
                     /label=stop
     misc_feature    1918..2657
                     /label=BBa_E0031
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg
      241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      421 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      661 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagaact
      961 taaattacga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1021 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
     1081 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc
     1141 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     1201 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     1261 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     1321 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     1381 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     1441 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     1501 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     1561 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     1621 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1681 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     1741 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1801 ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1861 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctacgttt actagaggaa
     1921 agaggagaaa ttaagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1981 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2041 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2101 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta
     2161 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2221 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2281 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2341 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2401 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2461 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2521 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     2581 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2641 cgagctgtac aagtaat
//