##gff-version 3
##sequence-region BBa_J70308 1 2657
BBa_J70308	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J70308	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J70308	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J70308	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J70308	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J70308	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J70308	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J70308	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J70308	.	engineered_region	957	1909	.	+	0	ID=BBa_J70307;Name=BBa_J70307
BBa_J70308	.	engineered_region	969	1012	.	+	0	ID=annotation2003943;Name=B0012;Parent=BBa_J70307
BBa_J70308	.	engineered_region	1021	1075	.	+	0	ID=annotation2003944;Name=R0011;Parent=BBa_J70307
BBa_J70308	.	sequence_feature	1816	1823	.	+	0	ID=annotation2003947;Name=scar;Parent=BBa_J70307
BBa_J70308	.	sequence_feature	1076	1083	.	+	0	ID=annotation2003945;Name=scar;Parent=BBa_J70307
BBa_J70308	.	engineered_region	1824	1906	.	+	0	ID=annotation2003948;Name=B0012;Parent=BBa_J70307
BBa_J70308	.	sequence_feature	1893	1906	.	+	0	ID=annotation2003949;Name=PsrI;Parent=BBa_J70307
BBa_J70308	.	engineered_region	1084	1815	.	+	0	ID=annotation2003946;Name=J06504 mCherry ;Parent=BBa_J70307
BBa_J70308	.	sequence_feature	966	968	.	+	0	ID=annotation2003942;Name=PsrI;Parent=BBa_J70307
BBa_J70308	.	sequence_feature	957	959	.	+	0	ID=annotation2003940;Name=PsrI ;Parent=BBa_J70307
BBa_J70308	.	sequence_feature	1013	1020	.	+	0	ID=annotation2003941;Name=scar;Parent=BBa_J70307
BBa_J70308	.	engineered_region	209	948	.	+	0	ID=BBa_E0021;Name=BBa_E0021
BBa_J70308	.	stop_codon	945	947	.	+	0	ID=annotation353638;Name=stop;Parent=BBa_E0021
BBa_J70308	.	CDS	228	944	.	+	0	ID=annotation353637;Name=ECFP;Parent=BBa_E0021
BBa_J70308	.	ribosome_entry_site	209	227	.	+	0	ID=annotation353636;Name=Clontech RBS;Parent=BBa_E0021
BBa_J70308	.	engineered_region	1918	2657	.	+	0	ID=BBa_E0031;Name=BBa_E0031
BBa_J70308	.	stop_codon	2654	2656	.	+	0	ID=annotation353785;Name=stop;Parent=BBa_E0031
BBa_J70308	.	ribosome_entry_site	1919	1937	.	+	0	ID=annotation353780;Name=Clontech RBS;Parent=BBa_E0031
BBa_J70308	.	CDS	1938	2653	.	+	0	ID=annotation353784;Name=EYFP;Parent=BBa_E0031
>BBa_J70308
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagaggaaagaggagaaattaagcatggtgagca
agggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgt
gtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtg
ccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacg
acttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagac
ccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggc
aacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggca
tcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccc
catcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgag
aagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaat
tactagagaacttaaattacgagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtg
agcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggt
gagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaac
ggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgacca
agggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccc
cgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggc
ggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaact
tcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacgg
cgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctac
aaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggact
acaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctacgtttactagaggaaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtg
cccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacct
acggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcgg
ctacggcctgcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggc
tacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgaca
ccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaa
ctacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaac
atcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccg
acaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagtt
cgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaat