BBa_J70310 1 BBa_J70310 Mesoplasma florum pBG7AU plasmid origin 2009-06-15T11:00:00Z 2015-05-08T01:08:21Z PCR from recircularized pBG7AU cut at the ClaI site. Original plasmid from Djordjevic01. Primers: GTTTCTTC GAATTC GCGGCCGC T TCTAGA Ggagtaaattctcctttttttgg,BG7AU-F GTTTCTTC CTGCAG CGGCCGC T ACTAGT Acaaaaaaaggagaatttactcc,BG7AU-R This is a biobricked version of the circular plasmid pBG7AU, Genbank NC_002569. The part is cut at the end of the Orf1 coding region, following the transcriptional terminator. This is similar to the location of the cut site used in the pKMK1 construction of Dybvig et al (King94) which formed their plasmid p2D4. false false _41_48_1_ 0 6 48 Not in stock false Unclear location for the cut site of the circular plasmid. false Tom Knight annotation2005557 1 orfA range2005557 1 414 1019 annotation2005558 1 terminator range2005558 1 1003 1034 annotation2011588 1 ClaI site range2011588 1 800 805 annotation2005556 1 orfB range2005556 1 196 354 BBa_J70310_sequence 1 ctcctttttttggggttagcgatagggggctactacgatagccccctaagagtgacttgtgacatgtgacgaaatggataaacaaatattatttttttgctagcatttgttgttgttttgctagcatttgttgttgttttgctagcatttgttaatgtttgttatataatgtttaatagaaaggaataacatataatggataagttagttaccttgagattagatgaacaacaacacgaaaaattaaaagaacaagcaaataagttagggatgactataagtggttatgttcgttatcttgttcttaaaagttctgaaatgaaaacaaaggaaagcaaaaaaaagaagctttagttctctaataagcttctttgtaaaacaaatattttttatcccttatattatagcaaataatgtcaactaatattaaaaaaagattttgaacgttattagtttatccggatagtgctccagaaaattgaaaagagattttagaccaaaatggagtagaatattttggagcattacacgataaagatgtcaatccggatggaacaattaaaaaagcgcattatcatatcgtgttagtttatagtggacccactacgtttaataacgttaaaacgctatgtaatacgctaaattcacctaaacctttacctttagacggtgttgggggaatgtggcgttatatgacacataaggacaacccggaaaaataccaatatgatgatagtattatttttaccggtaatggttttgatatttctaactataaagatctaagtaagaaagaaatatcgattataaagcaatcattattagacctaatagataacgaacttataactgagtattcggtttttataaggagagtaagagaattaggtatttccgaagcgtttgatatagcagctaacaacacaatattttttaataactacatcatttcctttaagttcgacttaaaaaacaaagcagaacaggaatatgaggaaatgactaaaaaaggggagtaaattctcctttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z