LOCUS       BBa_J70312               843 bp    DNA     linear       22-Nov-2024
DEFINITION  E0031 (RBS+EYFP), B0014 (terminator)
ACCESSION   BBa_J70312
VERSION     BBa_J70312.1  
FEATURES             Location/Qualifiers
     RBS             2..20
                     /label=Clontech RBS
     CDS             21..736
                     /label=EYFP
     misc_feature    737..739
                     /label=stop
     misc_feature    1..740
                     /label=BBa_E0031
     stem_loop       756..775
                     /label=T7 TE
     misc_feature    782..782
                     /label=stop
     polyA_site      776..789
                     /label=polya
     misc_feature    749..789
                     /label=BBa_B0012
     terminator      749..789
                     /label=BBa_B0012
     stem_loop       810..832
                     /label=stem_loop
     misc_feature    798..843
                     /label=BBa_B0011
     terminator      798..843
                     /label=BBa_B0011
ORIGIN
        1 gaaagaggag aaattaagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
       61 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      121 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      181 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg
      241 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      301 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      361 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      421 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      481 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      541 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      601 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
      661 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      721 ggacgagctg tacaagtaat tactagagtc acactggctc accttcgggt gggcctttct
      781 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
      841 ttt
//