LOCUS       BBa_J70316              1612 bp    DNA     linear       22-Nov-2024
DEFINITION  Calibration of YFP input to RFP (mcherry) output
ACCESSION   BBa_J70316
VERSION     BBa_J70316.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             64..82
                     /label=Clontech RBS
     CDS             83..798
                     /label=EYFP
     misc_feature    799..801
                     /label=stop
     misc_feature    63..802
                     /label=BBa_E0031
     CDS             829..1509
                     /label=MF-RED
     RBS             817..823
                     /label=RBS
     misc_feature    811..1509
                     /label=BBa_J70029
     stem_loop       1525..1544
                     /label=T7 TE
     misc_feature    1551..1551
                     /label=stop
     polyA_site      1545..1558
                     /label=polya
     misc_feature    1518..1558
                     /label=BBa_B0012
     terminator      1518..1558
                     /label=BBa_B0012
     stem_loop       1579..1601
                     /label=stem_loop
     misc_feature    1567..1612
                     /label=BBa_B0011
     terminator      1567..1612
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggaaagagg agaaattaag catggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagta attactagag tcacacagga aagactagat ggctagtagt
      841 gaggatataa tcaaagagtt tatgcgtttt aaagttcaca tggaaggttc agtaaacgga
      901 catgaatttg aaattgaggg agaaggagag ggtcgtccgt acgaaggtac acaaacagca
      961 aagttaaaag taacaaaagg aggaccttta ccatttgcat gggatattct ttctccacaa
     1021 tttatgtatg gtagtaaagc gtatgttaaa cacccggcag atataccaga ctatttaaag
     1081 ttgagttttc cggaaggatt caaatgggaa cgtgttatga attttgaaga tggtggtgtt
     1141 gttacagtta ctcaagatag tagcttacag gatggtgaat ttatttacaa agtaaaatta
     1201 cgtggtacta acttcccgag cgatggtcca gtaatgcaaa agaaaactat gggatgggag
     1261 gctagttctg aacgtatgta tcctgaagac ggtgctttaa aaggagaaat taaacaacgt
     1321 ttgaaactta aagatggtgg acactacgat gcagaagtta aaactacata taaagctaaa
     1381 aagcctgttc agcttccggg tgcttataat gtgaatataa aattagatat cacaagtcat
     1441 aacgaagatt atactattgt tgaacagtat gaaagagcag aaggaagaca ttctacagga
     1501 gcataataat actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1561 ctagagagag aatataaaaa gccagattat taatccggct tttttattat tt
//