LOCUS       BBa_J70318               905 bp    DNA     linear       22-Dec-2024
DEFINITION  Maximum YFP output (tetR, yfp, B0014)
ACCESSION   BBa_J70318
VERSION     BBa_J70318.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             64..82
                     /label=Clontech RBS
     CDS             83..798
                     /label=EYFP
     misc_feature    799..801
                     /label=stop
     misc_feature    63..802
                     /label=BBa_E0031
     stem_loop       818..837
                     /label=T7 TE
     misc_feature    844..844
                     /label=stop
     polyA_site      838..851
                     /label=polya
     misc_feature    811..851
                     /label=BBa_B0012
     terminator      811..851
                     /label=BBa_B0012
     stem_loop       872..894
                     /label=stem_loop
     misc_feature    860..905
                     /label=BBa_B0011
     terminator      860..905
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggaaagagg agaaattaag catggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagta attactagag tcacactggc tcaccttcgg gtgggccttt
      841 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat
      901 tattt
//