##gff-version 3
##sequence-region BBa_J70319 1 864
BBa_J70319	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J70319	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J70319	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J70319	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J70319	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J70319	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J70319	.	terminator	819	864	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_J70319	.	stem_loop	831	853	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J70319	.	engineered_region	819	864	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J70319	.	engineered_region	63	761	.	+	0	ID=BBa_J70029;Name=BBa_J70029
BBa_J70319	.	ribosome_entry_site	69	75	.	+	0	ID=annotation2002220;Name=RBS;Parent=BBa_J70029
BBa_J70319	.	CDS	81	761	.	+	0	ID=annotation2002219;Name=MF-RED;Parent=BBa_J70029
BBa_J70319	.	terminator	770	810	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J70319	.	engineered_region	770	810	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J70319	.	stop_codon	803	803	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J70319	.	polyA_site	797	810	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J70319	.	stem_loop	777	796	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_J70319
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagacta
gatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaa
tttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggac
ctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatat
accagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgtt
acagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcg
atggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaa
aggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaa
aagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattg
ttgaacagtatgaaagagcagaaggaagacattctacaggagcataataatactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt