LOCUS       BBa_J70320              2466 bp    DNA     linear       01-Jun-2024
DEFINITION  YFP, terminator insert position, mcherry
ACCESSION   BBa_J70320
VERSION     BBa_J70320.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             64..82
                     /label=Clontech RBS
     CDS             83..798
                     /label=EYFP
     misc_feature    799..801
                     /label=stop
     misc_feature    63..802
                     /label=BBa_E0031
     misc_feature    811..1656
                     /label=BBa_J70315
     CDS             1683..2363
                     /label=MF-RED
     RBS             1671..1677
                     /label=RBS
     misc_feature    1665..2363
                     /label=BBa_J70029
     stem_loop       2379..2398
                     /label=T7 TE
     misc_feature    2405..2405
                     /label=stop
     polyA_site      2399..2412
                     /label=polya
     misc_feature    2372..2412
                     /label=BBa_B0012
     terminator      2372..2412
                     /label=BBa_B0012
     stem_loop       2433..2455
                     /label=stem_loop
     misc_feature    2421..2466
                     /label=BBa_B0011
     terminator      2421..2466
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggaaagagg agaaattaag catggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagta attactagag gcctgaacgg cctttacaaa gaggagaaat
      841 actagatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      901 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      961 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     1021 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1081 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1141 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc
     1201 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1261 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     1321 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     1381 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     1441 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1501 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaataat
     1561 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1621 atctgttgtt tgtcggtgaa cgctctgaac ggcctttact agagtcacac aggaaagact
     1681 agatggctag tagtgaggat ataatcaaag agtttatgcg ttttaaagtt cacatggaag
     1741 gttcagtaaa cggacatgaa tttgaaattg agggagaagg agagggtcgt ccgtacgaag
     1801 gtacacaaac agcaaagtta aaagtaacaa aaggaggacc tttaccattt gcatgggata
     1861 ttctttctcc acaatttatg tatggtagta aagcgtatgt taaacacccg gcagatatac
     1921 cagactattt aaagttgagt tttccggaag gattcaaatg ggaacgtgtt atgaattttg
     1981 aagatggtgg tgttgttaca gttactcaag atagtagctt acaggatggt gaatttattt
     2041 acaaagtaaa attacgtggt actaacttcc cgagcgatgg tccagtaatg caaaagaaaa
     2101 ctatgggatg ggaggctagt tctgaacgta tgtatcctga agacggtgct ttaaaaggag
     2161 aaattaaaca acgtttgaaa cttaaagatg gtggacacta cgatgcagaa gttaaaacta
     2221 catataaagc taaaaagcct gttcagcttc cgggtgctta taatgtgaat ataaaattag
     2281 atatcacaag tcataacgaa gattatacta ttgttgaaca gtatgaaaga gcagaaggaa
     2341 gacattctac aggagcataa taatactaga gtcacactgg ctcaccttcg ggtgggcctt
     2401 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
     2461 ttattt
//