LOCUS BBa_J70320 2466 bp DNA linear 31-Oct-2024 DEFINITION YFP, terminator insert position, mcherry ACCESSION BBa_J70320 VERSION BBa_J70320.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 64..82 /label=Clontech RBS CDS 83..798 /label=EYFP misc_feature 799..801 /label=stop misc_feature 63..802 /label=BBa_E0031 misc_feature 811..1656 /label=BBa_J70315 CDS 1683..2363 /label=MF-RED RBS 1671..1677 /label=RBS misc_feature 1665..2363 /label=BBa_J70029 stem_loop 2379..2398 /label=T7 TE misc_feature 2405..2405 /label=stop polyA_site 2399..2412 /label=polya misc_feature 2372..2412 /label=BBa_B0012 terminator 2372..2412 /label=BBa_B0012 stem_loop 2433..2455 /label=stem_loop misc_feature 2421..2466 /label=BBa_B0011 terminator 2421..2466 /label=BBa_B0011 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 aggaaagagg agaaattaag catggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagta attactagag gcctgaacgg cctttacaaa gaggagaaat 841 actagatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct 901 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg 961 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc 1021 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga 1081 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg 1141 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc 1201 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc 1261 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg 1321 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg 1381 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca 1441 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt 1501 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaataat 1561 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 1621 atctgttgtt tgtcggtgaa cgctctgaac ggcctttact agagtcacac aggaaagact 1681 agatggctag tagtgaggat ataatcaaag agtttatgcg ttttaaagtt cacatggaag 1741 gttcagtaaa cggacatgaa tttgaaattg agggagaagg agagggtcgt ccgtacgaag 1801 gtacacaaac agcaaagtta aaagtaacaa aaggaggacc tttaccattt gcatgggata 1861 ttctttctcc acaatttatg tatggtagta aagcgtatgt taaacacccg gcagatatac 1921 cagactattt aaagttgagt tttccggaag gattcaaatg ggaacgtgtt atgaattttg 1981 aagatggtgg tgttgttaca gttactcaag atagtagctt acaggatggt gaatttattt 2041 acaaagtaaa attacgtggt actaacttcc cgagcgatgg tccagtaatg caaaagaaaa 2101 ctatgggatg ggaggctagt tctgaacgta tgtatcctga agacggtgct ttaaaaggag 2161 aaattaaaca acgtttgaaa cttaaagatg gtggacacta cgatgcagaa gttaaaacta 2221 catataaagc taaaaagcct gttcagcttc cgggtgctta taatgtgaat ataaaattag 2281 atatcacaag tcataacgaa gattatacta ttgttgaaca gtatgaaaga gcagaaggaa 2341 gacattctac aggagcataa taatactaga gtcacactgg ctcaccttcg ggtgggcctt 2401 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta 2461 ttattt //