LOCUS       BBa_J70321              2466 bp    DNA     linear       15-Nov-2024
DEFINITION  mcherry, terminator insert position, YFP
ACCESSION   BBa_J70321
VERSION     BBa_J70321.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             81..761
                     /label=MF-RED
     RBS             69..75
                     /label=RBS
     misc_feature    63..761
                     /label=BBa_J70029
     misc_feature    770..1615
                     /label=BBa_J70315
     RBS             1625..1643
                     /label=Clontech RBS
     CDS             1644..2359
                     /label=EYFP
     misc_feature    2360..2362
                     /label=stop
     misc_feature    1624..2363
                     /label=BBa_E0031
     stem_loop       2379..2398
                     /label=T7 TE
     misc_feature    2405..2405
                     /label=stop
     polyA_site      2399..2412
                     /label=polya
     misc_feature    2372..2412
                     /label=BBa_B0012
     terminator      2372..2412
                     /label=BBa_B0012
     stem_loop       2433..2455
                     /label=stem_loop
     misc_feature    2421..2466
                     /label=BBa_B0011
     terminator      2421..2466
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagactag atggctagta gtgaggatat aatcaaagag tttatgcgtt
      121 ttaaagttca catggaaggt tcagtaaacg gacatgaatt tgaaattgag ggagaaggag
      181 agggtcgtcc gtacgaaggt acacaaacag caaagttaaa agtaacaaaa ggaggacctt
      241 taccatttgc atgggatatt ctttctccac aatttatgta tggtagtaaa gcgtatgtta
      301 aacacccggc agatatacca gactatttaa agttgagttt tccggaagga ttcaaatggg
      361 aacgtgttat gaattttgaa gatggtggtg ttgttacagt tactcaagat agtagcttac
      421 aggatggtga atttatttac aaagtaaaat tacgtggtac taacttcccg agcgatggtc
      481 cagtaatgca aaagaaaact atgggatggg aggctagttc tgaacgtatg tatcctgaag
      541 acggtgcttt aaaaggagaa attaaacaac gtttgaaact taaagatggt ggacactacg
      601 atgcagaagt taaaactaca tataaagcta aaaagcctgt tcagcttccg ggtgcttata
      661 atgtgaatat aaaattagat atcacaagtc ataacgaaga ttatactatt gttgaacagt
      721 atgaaagagc agaaggaaga cattctacag gagcataata atactagagg cctgaacggc
      781 ctttacaaag aggagaaata ctagatggtg agcaagggcg aggaggataa catggccatc
      841 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      901 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      961 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1021 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1081 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1141 acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1201 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1261 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1321 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1381 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1441 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1501 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1561 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctgaacg gcctttacta
     1621 gaggaaagag gagaaattaa gcatggtgag caagggcgag gagctgttca ccggggtggt
     1681 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1741 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1801 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc
     1861 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1921 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1981 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2041 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2101 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2161 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2221 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     2281 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2341 catggacgag ctgtacaagt aattactaga gtcacactgg ctcaccttcg ggtgggcctt
     2401 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
     2461 ttattt
//