LOCUS       BBa_J70344              1610 bp    DNA     linear       16-Feb-2025
DEFINITION  Calibration of GFP input to RFP (mcherry) output
ACCESSION   BBa_J70344
VERSION     BBa_J70344.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     CDS             827..1507
                     /label=MF-RED
     RBS             815..821
                     /label=RBS
     misc_feature    809..1507
                     /label=BBa_J70029
     stem_loop       1523..1542
                     /label=T7 TE
     misc_feature    1549..1549
                     /label=stop
     polyA_site      1543..1556
                     /label=polya
     misc_feature    1516..1556
                     /label=BBa_B0012
     terminator      1516..1556
                     /label=BBa_B0012
     stem_loop       1577..1599
                     /label=stem_loop
     misc_feature    1565..1610
                     /label=BBa_B0011
     terminator      1565..1610
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagtc acacaggaaa gactagatgg ctagtagtga
      841 ggatataatc aaagagttta tgcgttttaa agttcacatg gaaggttcag taaacggaca
      901 tgaatttgaa attgagggag aaggagaggg tcgtccgtac gaaggtacac aaacagcaaa
      961 gttaaaagta acaaaaggag gacctttacc atttgcatgg gatattcttt ctccacaatt
     1021 tatgtatggt agtaaagcgt atgttaaaca cccggcagat ataccagact atttaaagtt
     1081 gagttttccg gaaggattca aatgggaacg tgttatgaat tttgaagatg gtggtgttgt
     1141 tacagttact caagatagta gcttacagga tggtgaattt atttacaaag taaaattacg
     1201 tggtactaac ttcccgagcg atggtccagt aatgcaaaag aaaactatgg gatgggaggc
     1261 tagttctgaa cgtatgtatc ctgaagacgg tgctttaaaa ggagaaatta aacaacgttt
     1321 gaaacttaaa gatggtggac actacgatgc agaagttaaa actacatata aagctaaaaa
     1381 gcctgttcag cttccgggtg cttataatgt gaatataaaa ttagatatca caagtcataa
     1441 cgaagattat actattgttg aacagtatga aagagcagaa ggaagacatt ctacaggagc
     1501 ataataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     1561 agagagagaa tataaaaagc cagattatta atccggcttt tttattattt
//