LOCUS       BBa_J70345              1610 bp    DNA     linear       25-Nov-2024
DEFINITION  Calibration of RFP (mcherry) input to GFP output
ACCESSION   BBa_J70345
VERSION     BBa_J70345.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             81..761
                     /label=MF-RED
     RBS             69..75
                     /label=RBS
     misc_feature    63..761
                     /label=BBa_J70029
     misc_feature    774..777
                     /label=conserved
     RBS             770..781
                     /label=BBa_B0034
     CDS             788..1507
                     /label=GFP protein
     CDS             788..1507
                     /label=BBa_E0040
     stem_loop       1523..1542
                     /label=T7 TE
     misc_feature    1549..1549
                     /label=stop
     polyA_site      1543..1556
                     /label=polya
     misc_feature    1516..1556
                     /label=BBa_B0012
     terminator      1516..1556
                     /label=BBa_B0012
     stem_loop       1577..1599
                     /label=stem_loop
     misc_feature    1565..1610
                     /label=BBa_B0011
     terminator      1565..1610
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagactag atggctagta gtgaggatat aatcaaagag tttatgcgtt
      121 ttaaagttca catggaaggt tcagtaaacg gacatgaatt tgaaattgag ggagaaggag
      181 agggtcgtcc gtacgaaggt acacaaacag caaagttaaa agtaacaaaa ggaggacctt
      241 taccatttgc atgggatatt ctttctccac aatttatgta tggtagtaaa gcgtatgtta
      301 aacacccggc agatatacca gactatttaa agttgagttt tccggaagga ttcaaatggg
      361 aacgtgttat gaattttgaa gatggtggtg ttgttacagt tactcaagat agtagcttac
      421 aggatggtga atttatttac aaagtaaaat tacgtggtac taacttcccg agcgatggtc
      481 cagtaatgca aaagaaaact atgggatggg aggctagttc tgaacgtatg tatcctgaag
      541 acggtgcttt aaaaggagaa attaaacaac gtttgaaact taaagatggt ggacactacg
      601 atgcagaagt taaaactaca tataaagcta aaaagcctgt tcagcttccg ggtgcttata
      661 atgtgaatat aaaattagat atcacaagtc ataacgaaga ttatactatt gttgaacagt
      721 atgaaagagc agaaggaaga cattctacag gagcataata atactagaga aagaggagaa
      781 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      841 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      901 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      961 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     1021 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     1081 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     1141 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     1201 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     1261 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1321 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1381 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1441 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1501 ataataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     1561 agagagagaa tataaaaagc cagattatta atccggcttt tttattattt
//