LOCUS BBa_J70347 1654 bp DNA linear 16-Dec-2024 DEFINITION GFP, terminator insert position ACCESSION BBa_J70347 VERSION BBa_J70347.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..800 /label=GFP protein CDS 81..800 /label=BBa_E0040 misc_feature 809..1654 /label=BBa_J70315 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 781 atgaactata caaataataa tactagaggc ctgaacggcc tttacaaaga ggagaaatac 841 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc 901 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag 961 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg 1021 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag 1081 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag 1141 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag 1201 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 1261 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac 1321 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac 1381 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac 1441 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 1501 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac 1561 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1621 ctgttgtttg tcggtgaacg ctctgaacgg cctt //