LOCUS       BBa_J70349              2464 bp    DNA     linear       25-Nov-2024
DEFINITION  promoter, GFP, terminator insert position, mcherry
ACCESSION   BBa_J70349
VERSION     BBa_J70349.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     misc_feature    809..1654
                     /label=BBa_J70315
     CDS             1681..2361
                     /label=MF-RED
     RBS             1669..1675
                     /label=RBS
     misc_feature    1663..2361
                     /label=BBa_J70029
     stem_loop       2377..2396
                     /label=T7 TE
     misc_feature    2403..2403
                     /label=stop
     polyA_site      2397..2410
                     /label=polya
     misc_feature    2370..2410
                     /label=BBa_B0012
     terminator      2370..2410
                     /label=BBa_B0012
     stem_loop       2431..2453
                     /label=stem_loop
     misc_feature    2419..2464
                     /label=BBa_B0011
     terminator      2419..2464
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagaggc ctgaacggcc tttacaaaga ggagaaatac
      841 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      901 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      961 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     1021 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     1081 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     1141 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag
     1201 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1261 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     1321 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     1381 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     1441 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1501 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac
     1561 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1621 ctgttgtttg tcggtgaacg ctctgaacgg cctttactag agtcacacag gaaagactag
     1681 atggctagta gtgaggatat aatcaaagag tttatgcgtt ttaaagttca catggaaggt
     1741 tcagtaaacg gacatgaatt tgaaattgag ggagaaggag agggtcgtcc gtacgaaggt
     1801 acacaaacag caaagttaaa agtaacaaaa ggaggacctt taccatttgc atgggatatt
     1861 ctttctccac aatttatgta tggtagtaaa gcgtatgtta aacacccggc agatatacca
     1921 gactatttaa agttgagttt tccggaagga ttcaaatggg aacgtgttat gaattttgaa
     1981 gatggtggtg ttgttacagt tactcaagat agtagcttac aggatggtga atttatttac
     2041 aaagtaaaat tacgtggtac taacttcccg agcgatggtc cagtaatgca aaagaaaact
     2101 atgggatggg aggctagttc tgaacgtatg tatcctgaag acggtgcttt aaaaggagaa
     2161 attaaacaac gtttgaaact taaagatggt ggacactacg atgcagaagt taaaactaca
     2221 tataaagcta aaaagcctgt tcagcttccg ggtgcttata atgtgaatat aaaattagat
     2281 atcacaagtc ataacgaaga ttatactatt gttgaacagt atgaaagagc agaaggaaga
     2341 cattctacag gagcataata atactagagt cacactggct caccttcggg tgggcctttc
     2401 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2461 attt
//