LOCUS       BBa_J70350              2464 bp    DNA     linear       13-Jun-2024
DEFINITION  promoter, mcherry, terminator insert position, GFP
ACCESSION   BBa_J70350
VERSION     BBa_J70350.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             81..761
                     /label=MF-RED
     RBS             69..75
                     /label=RBS
     misc_feature    63..761
                     /label=BBa_J70029
     misc_feature    770..1615
                     /label=BBa_J70315
     misc_feature    1628..1631
                     /label=conserved
     RBS             1624..1635
                     /label=BBa_B0034
     CDS             1642..2361
                     /label=GFP protein
     CDS             1642..2361
                     /label=BBa_E0040
     stem_loop       2377..2396
                     /label=T7 TE
     misc_feature    2403..2403
                     /label=stop
     polyA_site      2397..2410
                     /label=polya
     misc_feature    2370..2410
                     /label=BBa_B0012
     terminator      2370..2410
                     /label=BBa_B0012
     stem_loop       2431..2453
                     /label=stem_loop
     misc_feature    2419..2464
                     /label=BBa_B0011
     terminator      2419..2464
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagactag atggctagta gtgaggatat aatcaaagag tttatgcgtt
      121 ttaaagttca catggaaggt tcagtaaacg gacatgaatt tgaaattgag ggagaaggag
      181 agggtcgtcc gtacgaaggt acacaaacag caaagttaaa agtaacaaaa ggaggacctt
      241 taccatttgc atgggatatt ctttctccac aatttatgta tggtagtaaa gcgtatgtta
      301 aacacccggc agatatacca gactatttaa agttgagttt tccggaagga ttcaaatggg
      361 aacgtgttat gaattttgaa gatggtggtg ttgttacagt tactcaagat agtagcttac
      421 aggatggtga atttatttac aaagtaaaat tacgtggtac taacttcccg agcgatggtc
      481 cagtaatgca aaagaaaact atgggatggg aggctagttc tgaacgtatg tatcctgaag
      541 acggtgcttt aaaaggagaa attaaacaac gtttgaaact taaagatggt ggacactacg
      601 atgcagaagt taaaactaca tataaagcta aaaagcctgt tcagcttccg ggtgcttata
      661 atgtgaatat aaaattagat atcacaagtc ataacgaaga ttatactatt gttgaacagt
      721 atgaaagagc agaaggaaga cattctacag gagcataata atactagagg cctgaacggc
      781 ctttacaaag aggagaaata ctagatggtg agcaagggcg aggaggataa catggccatc
      841 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      901 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      961 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1021 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1081 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1141 acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1201 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1261 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1321 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1381 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1441 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1501 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1561 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctgaacg gcctttacta
     1621 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
     1681 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     1741 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     1801 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     1861 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1921 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1981 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     2041 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     2101 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     2161 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     2221 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     2281 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     2341 gatgaactat acaaataata atactagagt cacactggct caccttcggg tgggcctttc
     2401 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2461 attt
//