BBa_J70351
type: ComponentDefinition
persistent identity: BBa J70351
display id: BBa_J70351
version: 1
was generated by: iGEM to SBOL conversion
description: tetT promoter, GFP, B1001, mcherry
created: 2009-06-21T11:00:00Z
modified: 2015-05-08T01:08:21Z
mutable provenance: J70324, B1001, J70314
owned by: james
owned by: myers
mutable description: J70324(gfp reporter frag), B1001 (terminator), J70314 (mcherry translational unit)
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 1201
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Julie Norville
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_R0040
component: BBa_E0040
component: BBa_B0034
component: BBa_J70029
component: BBa_B1001
component: BBa_B0011
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B1001
sequence annotation: BBa_J70029
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_J70351_sequence/1

BBa_B0012
type: Component
persistent identity: component2006327
display id: component2006327
version: 1
top level: BBa_J70351
definition: BBa_B0012
access: public

BBa_R0040
type: Component
persistent identity: component2006310
display id: component2006310
version: 1
top level: BBa_J70351
definition: p(tetR)
access: public

BBa_E0040
type: Component
persistent identity: component2006318
display id: component2006318
version: 1
top level: BBa_J70351
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2006316
display id: component2006316
version: 1
top level: BBa_J70351
definition: BBa_B0034
access: public

BBa_J70029
type: Component
persistent identity: component2006326
display id: component2006326
version: 1
top level: BBa_J70351
definition: BBa_J70029
access: public

BBa_B1001
type: Component
persistent identity: component2006323
display id: component2006323
version: 1
top level: BBa_J70351
definition: BBa_B1001
access: public

BBa_B0011
type: Component
persistent identity: component2006331
display id: component2006331
version: 1
top level: BBa_J70351
definition: BBa_B0011
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2006310
display id: annotation2006310
version: 1
top level: BBa_J70351
location: https://synbiohub.org/public/igem/BBa_J70351/annotation2006310/range2006310/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J70351/annotation2006310/range2006310/1
type: Range
persistent identity: range2006310
display id: range2006310
version: 1
top level: BBa_J70351
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2006316
display id: annotation2006316
version: 1
top level: BBa_J70351
location: https://synbiohub.org/public/igem/BBa_J70351/annotation2006316/range2006316/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J70351/annotation2006316/range2006316/1
type: Range
persistent identity: range2006316
display id: range2006316
version: 1
top level: BBa_J70351
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2006318
display id: annotation2006318
version: 1
top level: BBa_J70351
location: https://synbiohub.org/public/igem/BBa_J70351/annotation2006318/range2006318/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J70351/annotation2006318/range2006318/1
type: Range
persistent identity: range2006318
display id: range2006318
version: 1
top level: BBa_J70351
direction: direction/reverse
start: 81
end: 800
orientation: inline

BBa_B1001
type: SequenceAnnotation
persistent identity: annotation2006323
display id: annotation2006323
version: 1
top level: BBa_J70351
location: https://synbiohub.org/public/igem/BBa_J70351/annotation2006323/range2006323/1
component: BBa_B1001

https://synbiohub.org/public/igem/BBa_J70351/annotation2006323/range2006323/1
type: Range
persistent identity: range2006323
display id: range2006323
version: 1
top level: BBa_J70351
direction: direction/reverse
start: 809
end: 842
orientation: inline

BBa_J70029
type: SequenceAnnotation
persistent identity: annotation2006326
display id: annotation2006326
version: 1
top level: BBa_J70351
location: https://synbiohub.org/public/igem/BBa_J70351/annotation2006326/range2006326/1
component: BBa_J70029

https://synbiohub.org/public/igem/BBa_J70351/annotation2006326/range2006326/1
type: Range
persistent identity: range2006326
display id: range2006326
version: 1
top level: BBa_J70351
direction: direction/reverse
start: 851
end: 1549
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2006327
display id: annotation2006327
version: 1
top level: BBa_J70351
location: https://synbiohub.org/public/igem/BBa_J70351/annotation2006327/range2006327/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J70351/annotation2006327/range2006327/1
type: Range
persistent identity: range2006327
display id: range2006327
version: 1
top level: BBa_J70351
direction: direction/forward
start: 1558
end: 1598
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2006331
display id: annotation2006331
version: 1
top level: BBa_J70351
location: https://synbiohub.org/public/igem/BBa_J70351/annotation2006331/range2006331/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_J70351/annotation2006331/range2006331/1
type: Range
persistent identity: range2006331
display id: range2006331
version: 1
top level: BBa_J70351
direction: direction/forward
start: 1607
end: 1652
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B1001
type: ComponentDefinition
persistent identity: BBa B1001
display id: BBa_B1001
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artifical, small, %T~=90)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artificial terminator, estimated %T>90
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Poly T tail of 9 residues for high %T
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1001
sequence annotation: PolyA tail
sequence annotation: stem loop
sequence annotation: Poly A tail
sequence: https://synbiohub.org/public/igem/BBa_B1001_sequence/1

B1001
type: SequenceAnnotation
persistent identity: annotation1898382
display id: annotation1898382
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B1001/annotation1898382/range1898382/1
type: Range
persistent identity: range1898382
display id: range1898382
version: 1
top level: BBa_B1001
direction: direction/forward
start: 1
end: 34
orientation: inline

PolyA tail
type: SequenceAnnotation
persistent identity: annotation1898381
display id: annotation1898381
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1001/annotation1898381/range1898381/1
type: Range
persistent identity: range1898381
display id: range1898381
version: 1
top level: BBa_B1001
direction: direction/reverse
start: 1
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898379
display id: annotation1898379
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1001/annotation1898379/range1898379/1
type: Range
persistent identity: range1898379
display id: range1898379
version: 1
top level: BBa_B1001
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898380
display id: annotation1898380
version: 1
top level: BBa_B1001
location: https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1001/annotation1898380/range1898380/1
type: Range
persistent identity: range1898380
display id: range1898380
version: 1
top level: BBa_B1001
direction: direction/reverse
start: 26
end: 34
orientation: inline

BBa_J70029
type: ComponentDefinition
persistent identity: BBa J70029
display id: BBa_J70029
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + mCherry gene, optimized for expression in Me. florum and E. coli
created: 2009-03-27T12:00:00Z
modified: 2015-05-08T01:08:20Z
mutable provenance: Synthesized by DNA 2.0, designed with Gene Designer. Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.
owned by: james
owned by: myers
mutable description: RBS + mCherry gene, optimized for expression in Me. florum and E. coli.
discontinued: false
dominant: true
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.
star: false
creator: Tom Knight
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: MF-RED
sequence: https://synbiohub.org/public/igem/BBa_J70029_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2002220
display id: annotation2002220
version: 1
top level: BBa_J70029
location: https://synbiohub.org/public/igem/BBa_J70029/annotation2002220/range2002220/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J70029/annotation2002220/range2002220/1
type: Range
persistent identity: range2002220
display id: range2002220
version: 1
top level: BBa_J70029
direction: direction/forward
start: 7
end: 13
orientation: inline

MF-RED
type: SequenceAnnotation
persistent identity: annotation2002219
display id: annotation2002219
version: 1
top level: BBa_J70029
location: https://synbiohub.org/public/igem/BBa_J70029/annotation2002219/range2002219/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J70029/annotation2002219/range2002219/1
type: Range
persistent identity: range2002219
display id: range2002219
version: 1
top level: BBa_J70029
direction: direction/forward
start: 19
end: 699
orientation: inline

https://synbiohub.org/public/igem/BBa_J70351_sequence/1
type: Sequence
persistent identity: BBa J70351 sequence
display id: BBa_J70351_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaaaaaaaaccccgcttcggcggggttttttttttactagagtcacacaggaaagactagatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaatttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggacctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatataccagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcgatggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaaaggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaaaagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattgttgaacagtatgaaagagcagaaggaagacattctacaggagcataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1001_sequence/1
type: Sequence
persistent identity: BBa B1001 sequence
display id: BBa_B1001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcttcggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70029_sequence/1
type: Sequence
persistent identity: BBa J70029 sequence
display id: BBa_J70029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagactagatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaatttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggacctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatataccagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcgatggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaaaggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaaaagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattgttgaacagtatgaaagagcagaaggaagacattctacaggagcataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z