##gff-version 3
##sequence-region BBa_J70355 1 1652
BBa_J70355	.	terminator	809	842	.	+	0	ID=BBa_B1003;Name=BBa_B1003
BBa_J70355	.	polyA_site	834	838	.	+	0	ID=annotation1898419;Name=PolyA;Parent=BBa_B1003
BBa_J70355	.	polyA_site	813	817	.	+	0	ID=annotation1898418;Name=PolyA;Parent=BBa_B1003
BBa_J70355	.	stem_loop	818	833	.	+	0	ID=annotation1898417;Name=stem loop;Parent=BBa_B1003
BBa_J70355	.	engineered_region	809	842	.	+	0	ID=annotation1898416;Name=B1003;Parent=BBa_B1003
BBa_J70355	.	engineered_region	851	1549	.	+	0	ID=BBa_J70029;Name=BBa_J70029
BBa_J70355	.	ribosome_entry_site	857	863	.	+	0	ID=annotation2002220;Name=RBS;Parent=BBa_J70029
BBa_J70355	.	CDS	869	1549	.	+	0	ID=annotation2002219;Name=MF-RED;Parent=BBa_J70029
BBa_J70355	.	terminator	1558	1598	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J70355	.	stem_loop	1565	1584	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J70355	.	polyA_site	1585	1598	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J70355	.	stop_codon	1591	1591	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J70355	.	engineered_region	1558	1598	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J70355	.	CDS	81	800	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J70355	.	CDS	81	800	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J70355	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J70355	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J70355	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J70355	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J70355	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J70355	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J70355	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J70355	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J70355	.	terminator	1607	1652	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_J70355	.	engineered_region	1607	1652	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J70355	.	stem_loop	1619	1641	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_J70355
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaataataatactagagcgccaaaaaccccgcttcggcggggtttttccgctactagagtcacacaggaaagactaga
tggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaatt
tgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggacct
ttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatatac
cagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgttac
agttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcgat
ggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaaag
gagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaaaa
gcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattgtt
gaacagtatgaaagagcagaaggaagacattctacaggagcataataatactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt