LOCUS       BBa_J70356              1652 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR promoter, mcherry, B1003, GFP
ACCESSION   BBa_J70356
VERSION     BBa_J70356.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             81..761
                     /label=MF-RED
     RBS             69..75
                     /label=RBS
     misc_feature    63..761
                     /label=BBa_J70029
     misc_feature    770..803
                     /label=B1003
     stem_loop       779..794
                     /label=stem loop
     polyA_site      774..778
                     /label=PolyA
     polyA_site      795..799
                     /label=PolyA
     terminator      770..803
                     /label=BBa_B1003
     misc_feature    816..819
                     /label=conserved
     RBS             812..823
                     /label=BBa_B0034
     CDS             830..1549
                     /label=GFP protein
     CDS             830..1549
                     /label=BBa_E0040
     stem_loop       1565..1584
                     /label=T7 TE
     misc_feature    1591..1591
                     /label=stop
     polyA_site      1585..1598
                     /label=polya
     misc_feature    1558..1598
                     /label=BBa_B0012
     terminator      1558..1598
                     /label=BBa_B0012
     stem_loop       1619..1641
                     /label=stem_loop
     misc_feature    1607..1652
                     /label=BBa_B0011
     terminator      1607..1652
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagactag atggctagta gtgaggatat aatcaaagag tttatgcgtt
      121 ttaaagttca catggaaggt tcagtaaacg gacatgaatt tgaaattgag ggagaaggag
      181 agggtcgtcc gtacgaaggt acacaaacag caaagttaaa agtaacaaaa ggaggacctt
      241 taccatttgc atgggatatt ctttctccac aatttatgta tggtagtaaa gcgtatgtta
      301 aacacccggc agatatacca gactatttaa agttgagttt tccggaagga ttcaaatggg
      361 aacgtgttat gaattttgaa gatggtggtg ttgttacagt tactcaagat agtagcttac
      421 aggatggtga atttatttac aaagtaaaat tacgtggtac taacttcccg agcgatggtc
      481 cagtaatgca aaagaaaact atgggatggg aggctagttc tgaacgtatg tatcctgaag
      541 acggtgcttt aaaaggagaa attaaacaac gtttgaaact taaagatggt ggacactacg
      601 atgcagaagt taaaactaca tataaagcta aaaagcctgt tcagcttccg ggtgcttata
      661 atgtgaatat aaaattagat atcacaagtc ataacgaaga ttatactatt gttgaacagt
      721 atgaaagagc agaaggaaga cattctacag gagcataata atactagagc gccaaaaacc
      781 ccgcttcggc ggggtttttc cgctactaga gaaagaggag aaatactaga tgcgtaaagg
      841 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      901 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      961 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     1021 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
     1081 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1141 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     1201 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     1261 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     1321 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     1381 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     1441 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     1501 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagtca
     1561 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
     1621 gccagattat taatccggct tttttattat tt
//