LOCUS       BBa_J70357              1652 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR promoter, GFP, B1004, mcherry
ACCESSION   BBa_J70357
VERSION     BBa_J70357.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     misc_feature    809..842
                     /label=B1004
     stem_loop       818..833
                     /label=stem loop
     polyA_site      814..817
                     /label=PolyA
     polyA_site      834..837
                     /label=PolyA
     terminator      809..842
                     /label=BBa_B1004
     CDS             869..1549
                     /label=MF-RED
     RBS             857..863
                     /label=RBS
     misc_feature    851..1549
                     /label=BBa_J70029
     stem_loop       1565..1584
                     /label=T7 TE
     misc_feature    1591..1591
                     /label=stop
     polyA_site      1585..1598
                     /label=polya
     misc_feature    1558..1598
                     /label=BBa_B0012
     terminator      1558..1598
                     /label=BBa_B0012
     stem_loop       1619..1641
                     /label=stem_loop
     misc_feature    1607..1652
                     /label=BBa_B0011
     terminator      1607..1652
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagcg ccgaaaaccc cgcttcggcg gggttttgcc
      841 gctactagag tcacacagga aagactagat ggctagtagt gaggatataa tcaaagagtt
      901 tatgcgtttt aaagttcaca tggaaggttc agtaaacgga catgaatttg aaattgaggg
      961 agaaggagag ggtcgtccgt acgaaggtac acaaacagca aagttaaaag taacaaaagg
     1021 aggaccttta ccatttgcat gggatattct ttctccacaa tttatgtatg gtagtaaagc
     1081 gtatgttaaa cacccggcag atataccaga ctatttaaag ttgagttttc cggaaggatt
     1141 caaatgggaa cgtgttatga attttgaaga tggtggtgtt gttacagtta ctcaagatag
     1201 tagcttacag gatggtgaat ttatttacaa agtaaaatta cgtggtacta acttcccgag
     1261 cgatggtcca gtaatgcaaa agaaaactat gggatgggag gctagttctg aacgtatgta
     1321 tcctgaagac ggtgctttaa aaggagaaat taaacaacgt ttgaaactta aagatggtgg
     1381 acactacgat gcagaagtta aaactacata taaagctaaa aagcctgttc agcttccggg
     1441 tgcttataat gtgaatataa aattagatat cacaagtcat aacgaagatt atactattgt
     1501 tgaacagtat gaaagagcag aaggaagaca ttctacagga gcataataat actagagtca
     1561 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
     1621 gccagattat taatccggct tttttattat tt
//