LOCUS       BBa_J70361              1657 bp    DNA     linear       23-Dec-2024
DEFINITION  TetR promoter, GFP, B1006, mcherry
ACCESSION   BBa_J70361
VERSION     BBa_J70361.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     misc_feature    809..847
                     /label=B1006
     stem_loop       818..839
                     /label=modified thr terminator
     polyA_site      840..847
                     /label=PolyA
     polyA_site      809..817
                     /label=PolyA
     terminator      809..847
                     /label=BBa_B1006
     CDS             874..1554
                     /label=MF-RED
     RBS             862..868
                     /label=RBS
     misc_feature    856..1554
                     /label=BBa_J70029
     stem_loop       1570..1589
                     /label=T7 TE
     misc_feature    1596..1596
                     /label=stop
     polyA_site      1590..1603
                     /label=polya
     misc_feature    1563..1603
                     /label=BBa_B0012
     terminator      1563..1603
                     /label=BBa_B0012
     stem_loop       1624..1646
                     /label=stem_loop
     misc_feature    1612..1657
                     /label=BBa_B0011
     terminator      1612..1657
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagaa aaaaaaaccc cgcccctgac agggcggggt
      841 ttttttttac tagagtcaca caggaaagac tagatggcta gtagtgagga tataatcaaa
      901 gagtttatgc gttttaaagt tcacatggaa ggttcagtaa acggacatga atttgaaatt
      961 gagggagaag gagagggtcg tccgtacgaa ggtacacaaa cagcaaagtt aaaagtaaca
     1021 aaaggaggac ctttaccatt tgcatgggat attctttctc cacaatttat gtatggtagt
     1081 aaagcgtatg ttaaacaccc ggcagatata ccagactatt taaagttgag ttttccggaa
     1141 ggattcaaat gggaacgtgt tatgaatttt gaagatggtg gtgttgttac agttactcaa
     1201 gatagtagct tacaggatgg tgaatttatt tacaaagtaa aattacgtgg tactaacttc
     1261 ccgagcgatg gtccagtaat gcaaaagaaa actatgggat gggaggctag ttctgaacgt
     1321 atgtatcctg aagacggtgc tttaaaagga gaaattaaac aacgtttgaa acttaaagat
     1381 ggtggacact acgatgcaga agttaaaact acatataaag ctaaaaagcc tgttcagctt
     1441 ccgggtgctt ataatgtgaa tataaaatta gatatcacaa gtcataacga agattatact
     1501 attgttgaac agtatgaaag agcagaagga agacattcta caggagcata ataatactag
     1561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat
     1621 aaaaagccag attattaatc cggctttttt attattt
//