LOCUS       BBa_J70362              1657 bp    DNA     linear       01-Jun-2024
DEFINITION  TetR promoter, mcherry, B1006, GFP
ACCESSION   BBa_J70362
VERSION     BBa_J70362.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             81..761
                     /label=MF-RED
     RBS             69..75
                     /label=RBS
     misc_feature    63..761
                     /label=BBa_J70029
     misc_feature    770..808
                     /label=B1006
     stem_loop       779..800
                     /label=modified thr terminator
     polyA_site      801..808
                     /label=PolyA
     polyA_site      770..778
                     /label=PolyA
     terminator      770..808
                     /label=BBa_B1006
     misc_feature    821..824
                     /label=conserved
     RBS             817..828
                     /label=BBa_B0034
     CDS             835..1554
                     /label=GFP protein
     CDS             835..1554
                     /label=BBa_E0040
     stem_loop       1570..1589
                     /label=T7 TE
     misc_feature    1596..1596
                     /label=stop
     polyA_site      1590..1603
                     /label=polya
     misc_feature    1563..1603
                     /label=BBa_B0012
     terminator      1563..1603
                     /label=BBa_B0012
     stem_loop       1624..1646
                     /label=stem_loop
     misc_feature    1612..1657
                     /label=BBa_B0011
     terminator      1612..1657
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagactag atggctagta gtgaggatat aatcaaagag tttatgcgtt
      121 ttaaagttca catggaaggt tcagtaaacg gacatgaatt tgaaattgag ggagaaggag
      181 agggtcgtcc gtacgaaggt acacaaacag caaagttaaa agtaacaaaa ggaggacctt
      241 taccatttgc atgggatatt ctttctccac aatttatgta tggtagtaaa gcgtatgtta
      301 aacacccggc agatatacca gactatttaa agttgagttt tccggaagga ttcaaatggg
      361 aacgtgttat gaattttgaa gatggtggtg ttgttacagt tactcaagat agtagcttac
      421 aggatggtga atttatttac aaagtaaaat tacgtggtac taacttcccg agcgatggtc
      481 cagtaatgca aaagaaaact atgggatggg aggctagttc tgaacgtatg tatcctgaag
      541 acggtgcttt aaaaggagaa attaaacaac gtttgaaact taaagatggt ggacactacg
      601 atgcagaagt taaaactaca tataaagcta aaaagcctgt tcagcttccg ggtgcttata
      661 atgtgaatat aaaattagat atcacaagtc ataacgaaga ttatactatt gttgaacagt
      721 atgaaagagc agaaggaaga cattctacag gagcataata atactagaga aaaaaaaacc
      781 ccgcccctga cagggcgggg ttttttttta ctagagaaag aggagaaata ctagatgcgt
      841 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      901 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      961 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1021 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
     1081 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1141 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1201 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1261 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1321 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1381 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1441 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1501 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat
     1621 aaaaagccag attattaatc cggctttttt attattt
//