LOCUS       BBa_J70363              1658 bp    DNA     linear       23-May-2024
DEFINITION  TetR promoter, GFP, B1007, mcherry
ACCESSION   BBa_J70363
VERSION     BBa_J70363.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     misc_feature    809..848
                     /label=B1007
     stem_loop       818..839
                     /label=modified thr
     polyA_site      812..817
                     /label=PolyA
     polyA_site      840..845
                     /label=PolyA
     terminator      809..848
                     /label=BBa_B1007
     CDS             875..1555
                     /label=MF-RED
     RBS             863..869
                     /label=RBS
     misc_feature    857..1555
                     /label=BBa_J70029
     stem_loop       1571..1590
                     /label=T7 TE
     misc_feature    1597..1597
                     /label=stop
     polyA_site      1591..1604
                     /label=polya
     misc_feature    1564..1604
                     /label=BBa_B0012
     terminator      1564..1604
                     /label=BBa_B0012
     stem_loop       1625..1647
                     /label=stem_loop
     misc_feature    1613..1658
                     /label=BBa_B0011
     terminator      1613..1658
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagcg caaaaaaccc cgcccctgac agggcggggt
      841 tttttcgcta ctagagtcac acaggaaaga ctagatggct agtagtgagg atataatcaa
      901 agagtttatg cgttttaaag ttcacatgga aggttcagta aacggacatg aatttgaaat
      961 tgagggagaa ggagagggtc gtccgtacga aggtacacaa acagcaaagt taaaagtaac
     1021 aaaaggagga cctttaccat ttgcatggga tattctttct ccacaattta tgtatggtag
     1081 taaagcgtat gttaaacacc cggcagatat accagactat ttaaagttga gttttccgga
     1141 aggattcaaa tgggaacgtg ttatgaattt tgaagatggt ggtgttgtta cagttactca
     1201 agatagtagc ttacaggatg gtgaatttat ttacaaagta aaattacgtg gtactaactt
     1261 cccgagcgat ggtccagtaa tgcaaaagaa aactatggga tgggaggcta gttctgaacg
     1321 tatgtatcct gaagacggtg ctttaaaagg agaaattaaa caacgtttga aacttaaaga
     1381 tggtggacac tacgatgcag aagttaaaac tacatataaa gctaaaaagc ctgttcagct
     1441 tccgggtgct tataatgtga atataaaatt agatatcaca agtcataacg aagattatac
     1501 tattgttgaa cagtatgaaa gagcagaagg aagacattct acaggagcat aataatacta
     1561 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     1621 taaaaagcca gattattaat ccggcttttt tattattt
//