LOCUS       BBa_J70366              1658 bp    DNA     linear       14-May-2024
DEFINITION  TetR promoter, mcherry, B1010, GFP
ACCESSION   BBa_J70366
VERSION     BBa_J70366.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             81..761
                     /label=MF-RED
     RBS             69..75
                     /label=RBS
     misc_feature    63..761
                     /label=BBa_J70029
     misc_feature    770..809
                     /label=B1010
     stem_loop       779..800
                     /label=modified thr terminator
     polyA_site      776..778
                     /label=PolyA
     polyA_site      801..803
                     /label=PolyA
     terminator      770..809
                     /label=BBa_B1010
     misc_feature    822..825
                     /label=conserved
     RBS             818..829
                     /label=BBa_B0034
     CDS             836..1555
                     /label=GFP protein
     CDS             836..1555
                     /label=BBa_E0040
     stem_loop       1571..1590
                     /label=T7 TE
     misc_feature    1597..1597
                     /label=stop
     polyA_site      1591..1604
                     /label=polya
     misc_feature    1564..1604
                     /label=BBa_B0012
     terminator      1564..1604
                     /label=BBa_B0012
     stem_loop       1625..1647
                     /label=stem_loop
     misc_feature    1613..1658
                     /label=BBa_B0011
     terminator      1613..1658
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagactag atggctagta gtgaggatat aatcaaagag tttatgcgtt
      121 ttaaagttca catggaaggt tcagtaaacg gacatgaatt tgaaattgag ggagaaggag
      181 agggtcgtcc gtacgaaggt acacaaacag caaagttaaa agtaacaaaa ggaggacctt
      241 taccatttgc atgggatatt ctttctccac aatttatgta tggtagtaaa gcgtatgtta
      301 aacacccggc agatatacca gactatttaa agttgagttt tccggaagga ttcaaatggg
      361 aacgtgttat gaattttgaa gatggtggtg ttgttacagt tactcaagat agtagcttac
      421 aggatggtga atttatttac aaagtaaaat tacgtggtac taacttcccg agcgatggtc
      481 cagtaatgca aaagaaaact atgggatggg aggctagttc tgaacgtatg tatcctgaag
      541 acggtgcttt aaaaggagaa attaaacaac gtttgaaact taaagatggt ggacactacg
      601 atgcagaagt taaaactaca tataaagcta aaaagcctgt tcagcttccg ggtgcttata
      661 atgtgaatat aaaattagat atcacaagtc ataacgaaga ttatactatt gttgaacagt
      721 atgaaagagc agaaggaaga cattctacag gagcataata atactagagc gccgcaaacc
      781 ccgcccctga cagggcgggg tttcgccgct actagagaaa gaggagaaat actagatgcg
      841 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      901 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      961 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1021 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1081 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1141 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1201 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1261 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1321 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1381 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1441 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1501 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     1561 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     1621 taaaaagcca gattattaat ccggcttttt tattattt
//