##gff-version 3
##sequence-region BBa_J70366 1 1658
BBa_J70366	.	CDS	836	1555	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J70366	.	CDS	836	1555	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J70366	.	terminator	1613	1658	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_J70366	.	engineered_region	1613	1658	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J70366	.	stem_loop	1625	1647	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J70366	.	terminator	1564	1604	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J70366	.	stem_loop	1571	1590	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J70366	.	polyA_site	1591	1604	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J70366	.	stop_codon	1597	1597	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J70366	.	engineered_region	1564	1604	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J70366	.	terminator	770	809	.	+	0	ID=BBa_B1010;Name=BBa_B1010
BBa_J70366	.	engineered_region	770	809	.	+	0	ID=annotation1901013;Name=B1010;Parent=BBa_B1010
BBa_J70366	.	polyA_site	801	803	.	+	0	ID=annotation1901016;Name=PolyA;Parent=BBa_B1010
BBa_J70366	.	polyA_site	776	778	.	+	0	ID=annotation1901015;Name=PolyA;Parent=BBa_B1010
BBa_J70366	.	stem_loop	779	800	.	+	0	ID=annotation1901014;Name=modified thr terminator;Parent=BBa_B1010
BBa_J70366	.	ribosome_entry_site	818	829	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J70366	.	conserved_region	822	825	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J70366	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J70366	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J70366	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J70366	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J70366	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J70366	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J70366	.	engineered_region	63	761	.	+	0	ID=BBa_J70029;Name=BBa_J70029
BBa_J70366	.	ribosome_entry_site	69	75	.	+	0	ID=annotation2002220;Name=RBS;Parent=BBa_J70029
BBa_J70366	.	CDS	81	761	.	+	0	ID=annotation2002219;Name=MF-RED;Parent=BBa_J70029
>BBa_J70366
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagacta
gatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaa
tttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggac
ctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatat
accagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgtt
acagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcg
atggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaa
aggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaa
aagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattg
ttgaacagtatgaaagagcagaaggaagacattctacaggagcataataatactagagcgccgcaaaccccgcccctga
cagggcggggtttcgccgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttg
tcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaac
atacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttc
ggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaag
gttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtga
tacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatac
aactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacaca
acattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacc
agacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgag
tttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt