LOCUS BBa_J70402 3313 bp DNA linear 16-Dec-2024 DEFINITION pBG7AU (J70310) plus TetM (J70028) ACCESSION BBa_J70402 VERSION BBa_J70402.1 FEATURES Location/Qualifiers CDS 196..354 /label=orfB CDS 414..1019 /label=orfA stem_loop 1003..1034 /label=terminator misc_feature 800..805 /label=ClaI site misc_feature 1..1034 /label=BBa_J70310 RBS 1381..1387 /label=RBS stem_loop 1361..1372 /label=term stem_loop 1315..1362 /label=stem_loop stem_loop 1296..1323 /label=stem_loop stem_loop 1284..1314 /label=stem_loop RBS 1279..1282 /label=rbs promoter 1110..1115 /label=-10 promoter 1087..1092 /label=-35 misc_feature 1122..1122 /label=+1 CDS 1292..1378 /label=leader peptide promoter 1043..1387 /label=BBa_J70025 CDS 1394..3313 /label=tetM gene CDS 1394..3313 /label=BBa_J70024 ORIGIN 1 ctcctttttt tggggttagc gatagggggc tactacgata gccccctaag agtgacttgt 61 gacatgtgac gaaatggata aacaaatatt atttttttgc tagcatttgt tgttgttttg 121 ctagcatttg ttgttgtttt gctagcattt gttaatgttt gttatataat gtttaataga 181 aaggaataac atataatgga taagttagtt accttgagat tagatgaaca acaacacgaa 241 aaattaaaag aacaagcaaa taagttaggg atgactataa gtggttatgt tcgttatctt 301 gttcttaaaa gttctgaaat gaaaacaaag gaaagcaaaa aaaagaagct ttagttctct 361 aataagcttc tttgtaaaac aaatattttt tatcccttat attatagcaa ataatgtcaa 421 ctaatattaa aaaaagattt tgaacgttat tagtttatcc ggatagtgct ccagaaaatt 481 gaaaagagat tttagaccaa aatggagtag aatattttgg agcattacac gataaagatg 541 tcaatccgga tggaacaatt aaaaaagcgc attatcatat cgtgttagtt tatagtggac 601 ccactacgtt taataacgtt aaaacgctat gtaatacgct aaattcacct aaacctttac 661 ctttagacgg tgttggggga atgtggcgtt atatgacaca taaggacaac ccggaaaaat 721 accaatatga tgatagtatt atttttaccg gtaatggttt tgatatttct aactataaag 781 atctaagtaa gaaagaaata tcgattataa agcaatcatt attagaccta atagataacg 841 aacttataac tgagtattcg gtttttataa ggagagtaag agaattaggt atttccgaag 901 cgtttgatat agcagctaac aacacaatat tttttaataa ctacatcatt tcctttaagt 961 tcgacttaaa aaacaaagca gaacaggaat atgaggaaat gactaaaaaa ggggagtaaa 1021 ttctcctttt tttgtactag agggccagtc tacatgtact ctttttgata aaaaattgga 1081 gattccttta caaatatgct cttacgtgct attatttaag tgactattta aaaggagtta 1141 ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagcggg aacaaataat 1201 tagatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca 1261 tgtgattcta aagtatccag agaatatctg tatgctttgt atacctatgg ttatgcataa 1321 aaatcccagt gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat 1381 ggaggaatac tagatgaaaa tcataaatat cggtgtatta gctcacgttg atgcaggaaa 1441 aacaacatta actgaatcac ttttatataa ctctggtgca attactgaac ttggttcagt 1501 agataaaggt actactcgta ctgataatac attattagaa cgtcaacgtg gaatcacaat 1561 tcaaacaggt atcacatctt ttcaatggga aaatacaaaa gtaaatatta tagatacacc 1621 tggacacatg gatttccttg cagaagtata ccgtagtctt tcagtattag atggtgctat 1681 tttacttatc agcgctaaag atggagttca agctcaaact cgtatcttat ttcacgcatt 1741 acgtaaaatg ggtattccaa caattttctt tataaacaaa attgaccaaa acggaattga 1801 tttaagtaca gtttatcaag atatcaaaga aaaactttct gctgaaatcg ttattaaaca 1861 aaaagttgaa ttatacccaa acgtttgcgt aacaaatttt actgaatcag aacaatggga 1921 tacagttata gaaggtaatg atgatttatt agaaaaatac atgtcaggta aatcattaga 1981 agcattagaa ttagaacaag aagaaagtat tcgtttccaa aactgttctt tattcccttt 2041 ataccatgga agcgctaaaa gtaacatagg tattgataac ttaattgaag ttattactaa 2101 caaattttac tcttcaactc atcgtggtcc ttctgaatta tgcggtaacg ttttcaaaat 2161 tgaatataca aaaaaacgtc aacgtttagc ttatatacgt ctttatagtg gtgttttaca 2221 tttacgtgat agtgttcgtg ttagtgaaaa agaaaagatt aaagttacag aaatgtatac 2281 ttctattaac ggtgaattat gcaaaattga ccgtgcatat tcaggtgaaa ttgtaatttt 2341 acaaaacgaa tttcttaaac ttaatagtgt acttggtgac acaaaacttt taccacaacg 2401 taagaaaatt gaaaatccac acccattact tcaaacaaca gtagaaccaa gcaaacctga 2461 acaacgtgaa atgcttttag atgctctttt agaaattagt gactctgacc cacttttacg 2521 ttactatgta gattctacta ctcatgaaat tattctttct ttccttggta aagttcaaat 2581 ggaagttatt tctgcattat tacaagaaaa atatcatgtt gaaatcgaat taaaagaacc 2641 tactgtaatt tatatggaac gtccattaaa aaatgctgaa tatacaattc atattgaagt 2701 tccaccaaat ccattttggg cttctattgg tctttctgtt tctccacttc cacttggtag 2761 cggaatgcaa tatgaaagta gcgtaagttt aggttatctt aatcaaagtt tccaaaacgc 2821 agttatggaa ggtattcgtt acggttgcga acaaggttta tacggttgga atgttacaga 2881 ctgcaaaatc tgttttaagt atggacttta ctattcacct gtatcaacac ctgctgactt 2941 tcgtatgctt gcaccaattg ttttagaaca agttttaaag aaagctggaa ctgaactttt 3001 agaaccatac ctttctttta aaatctatgc accacaagaa tacttaagtc gtgcttataa 3061 cgatgcacct aaatactgtg ctaatattgt tgatactcaa ttaaagaaca acgaagtaat 3121 tttaagcgga gaaattcctg cacgttgtat tcaagaatat cgtagtgatt taacattttt 3181 cactaatgga cgttctgttt gcttaactga attaaaaggt tatcatgtta ctactggtga 3241 acctgtatgc caaccacgtc gtcctaatag tcgtattgat aaagttcgtt atatgttcaa 3301 caaaatcaca taa //