BBa_J70025 1 BBa_J70025 Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid. Upstream promoter for tetM gene; contains a short peptide leader, regulatory sequences which are poorly understood. Use in combination with J70024. false false _41_48_1_ 0 6 48 Not in stock true Alignment of the promoter with the coding region, preservation of the RBS sites. false Tom Knight annotation1999389 1 -35 range1999389 1 45 50 annotation1999383 1 term range1999383 1 319 330 annotation1999391 1 leader peptide range1999391 1 250 336 annotation1999388 1 -10 range1999388 1 68 73 annotation1999384 1 stem_loop range1999384 1 273 320 annotation1999386 1 stem_loop range1999386 1 242 272 annotation1999390 1 +1 range1999390 1 80 80 annotation1999385 1 stem_loop range1999385 1 254 281 annotation1999387 1 rbs range1999387 1 237 240 annotation1999382 1 RBS range1999382 1 339 345 BBa_J70310 1 BBa_J70310 Mesoplasma florum pBG7AU plasmid origin 2009-06-15T11:00:00Z 2015-05-08T01:08:21Z PCR from recircularized pBG7AU cut at the ClaI site. Original plasmid from Djordjevic01. Primers: GTTTCTTC GAATTC GCGGCCGC T TCTAGA Ggagtaaattctcctttttttgg,BG7AU-F GTTTCTTC CTGCAG CGGCCGC T ACTAGT Acaaaaaaaggagaatttactcc,BG7AU-R This is a biobricked version of the circular plasmid pBG7AU, Genbank NC_002569. The part is cut at the end of the Orf1 coding region, following the transcriptional terminator. This is similar to the location of the cut site used in the pKMK1 construction of Dybvig et al (King94) which formed their plasmid p2D4. false false _41_48_1_ 0 6 48 Not in stock false Unclear location for the cut site of the circular plasmid. false Tom Knight annotation2005556 1 orfB range2005556 1 196 354 annotation2011588 1 ClaI site range2011588 1 800 805 annotation2005557 1 orfA range2005557 1 414 1019 annotation2005558 1 terminator range2005558 1 1003 1034 BBa_J70024 1 BBa_J70024 TetM gene, recoded compromise E. coli & Mesoplasma florum 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding. The tetM antibiotic resistance gene synthesized by Geneart from a protein sequence derived from the Ureaplasma urealyticum tetM gene. Synthesized with a compromise codon usage table for expression in both E. coli and Mesoplasma florum. Highly active in both E. coli and Mesoplasma florum. false false _41_48_1_ 0 6 48 Not in stock true Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species. false Tom Knight annotation1982961 1 tetM gene range1982961 1 1 1920 BBa_J70402 1 BBa_J70402 pBG7AU (J70310) plus TetM (J70028) 2009-07-27T11:00:00Z 2015-05-08T01:08:22Z Constructed in Knight Lab Plasmid pBG7AU (J70310) for Mesoplasma florum combined with Tet resistance cassette (J70028). false false _41_ 0 5347 41 Not in stock false See J70310 false Kelly Drinkwater component2013825 1 BBa_J70025 component2013827 1 BBa_J70024 component2013814 1 BBa_J70310 annotation2013825 1 BBa_J70025 range2013825 1 1043 1387 annotation2013827 1 BBa_J70024 range2013827 1 1394 3313 annotation2013814 1 BBa_J70310 range2013814 1 1 1034 BBa_J70024_sequence 1 atgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa BBa_J70310_sequence 1 ctcctttttttggggttagcgatagggggctactacgatagccccctaagagtgacttgtgacatgtgacgaaatggataaacaaatattatttttttgctagcatttgttgttgttttgctagcatttgttgttgttttgctagcatttgttaatgtttgttatataatgtttaatagaaaggaataacatataatggataagttagttaccttgagattagatgaacaacaacacgaaaaattaaaagaacaagcaaataagttagggatgactataagtggttatgttcgttatcttgttcttaaaagttctgaaatgaaaacaaaggaaagcaaaaaaaagaagctttagttctctaataagcttctttgtaaaacaaatattttttatcccttatattatagcaaataatgtcaactaatattaaaaaaagattttgaacgttattagtttatccggatagtgctccagaaaattgaaaagagattttagaccaaaatggagtagaatattttggagcattacacgataaagatgtcaatccggatggaacaattaaaaaagcgcattatcatatcgtgttagtttatagtggacccactacgtttaataacgttaaaacgctatgtaatacgctaaattcacctaaacctttacctttagacggtgttgggggaatgtggcgttatatgacacataaggacaacccggaaaaataccaatatgatgatagtattatttttaccggtaatggttttgatatttctaactataaagatctaagtaagaaagaaatatcgattataaagcaatcattattagacctaatagataacgaacttataactgagtattcggtttttataaggagagtaagagaattaggtatttccgaagcgtttgatatagcagctaacaacacaatattttttaataactacatcatttcctttaagttcgacttaaaaaacaaagcagaacaggaatatgaggaaatgactaaaaaaggggagtaaattctcctttttttg BBa_J70025_sequence 1 ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaa BBa_J70402_sequence 1 ctcctttttttggggttagcgatagggggctactacgatagccccctaagagtgacttgtgacatgtgacgaaatggataaacaaatattatttttttgctagcatttgttgttgttttgctagcatttgttgttgttttgctagcatttgttaatgtttgttatataatgtttaatagaaaggaataacatataatggataagttagttaccttgagattagatgaacaacaacacgaaaaattaaaagaacaagcaaataagttagggatgactataagtggttatgttcgttatcttgttcttaaaagttctgaaatgaaaacaaaggaaagcaaaaaaaagaagctttagttctctaataagcttctttgtaaaacaaatattttttatcccttatattatagcaaataatgtcaactaatattaaaaaaagattttgaacgttattagtttatccggatagtgctccagaaaattgaaaagagattttagaccaaaatggagtagaatattttggagcattacacgataaagatgtcaatccggatggaacaattaaaaaagcgcattatcatatcgtgttagtttatagtggacccactacgtttaataacgttaaaacgctatgtaatacgctaaattcacctaaacctttacctttagacggtgttgggggaatgtggcgttatatgacacataaggacaacccggaaaaataccaatatgatgatagtattatttttaccggtaatggttttgatatttctaactataaagatctaagtaagaaagaaatatcgattataaagcaatcattattagacctaatagataacgaacttataactgagtattcggtttttataaggagagtaagagaattaggtatttccgaagcgtttgatatagcagctaacaacacaatattttttaataactacatcatttcctttaagttcgacttaaaaaacaaagcagaacaggaatatgaggaaatgactaaaaaaggggagtaaattctcctttttttgtactagagggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaatactagatgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z