LOCUS BBa_J70403 2747 bp DNA linear 31-Oct-2024 DEFINITION R0010.E0031.J70394.E0021 or J70397.E0021 ACCESSION BBa_J70403 VERSION BBa_J70403.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 210..228 /label=Clontech RBS CDS 229..944 /label=EYFP misc_feature 945..947 /label=stop misc_feature 209..948 /label=BBa_E0031 misc_feature 957..1999 /label=BBa_J70394 RBS 2008..2026 /label=Clontech RBS CDS 2027..2743 /label=ECFP misc_feature 2744..2746 /label=stop misc_feature 2008..2747 /label=BBa_E0021 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg 241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 421 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagcag cacgacttct 481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 661 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagatac 961 atgaacatgc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 1021 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 1081 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 1141 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatggc 1201 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt 1261 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca 1321 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc 1381 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta 1441 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg 1501 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt 1561 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg 1621 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa 1681 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat 1741 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac 1801 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc 1861 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa 1921 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1981 aacgctctct actagagtct actagaggaa agaggagaaa ttaagcatgg tgagcaaggg 2041 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 2101 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 2161 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 2221 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 2281 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 2341 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 2401 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 2461 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa 2521 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 2581 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 2641 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 2701 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaat //