LOCUS BBa_J70416 1738 bp DNA linear 31-Oct-2024 DEFINITION R0010.E0031.B1002.E0021 ACCESSION BBa_J70416 VERSION BBa_J70416.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 210..228 /label=Clontech RBS CDS 229..944 /label=EYFP misc_feature 945..947 /label=stop misc_feature 209..948 /label=BBa_E0031 misc_feature 957..990 /label=B1002 stem_loop 966..981 /label=stem loop polyA_site 981..987 /label=Poly A tail polyA_site 960..965 /label=Poly A tail terminator 957..990 /label=BBa_B1002 RBS 999..1017 /label=Clontech RBS CDS 1018..1734 /label=ECFP misc_feature 1735..1737 /label=stop misc_feature 999..1738 /label=BBa_E0021 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg 241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 421 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagcag cacgacttct 481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 661 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagcgca 961 aaaaaccccg cttcggcggg gttttttcgc tactagagga aagaggagaa attaagcatg 1021 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 1081 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 1141 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 1201 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag 1261 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 1321 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 1381 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 1441 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc 1501 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 1561 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 1621 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 1681 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaat //