LOCUS BBa_J70420 1743 bp DNA linear 31-Oct-2024 DEFINITION R0010.E0031.B1006.E0021, Terminator test for B1006 ACCESSION BBa_J70420 VERSION BBa_J70420.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 210..228 /label=Clontech RBS CDS 229..944 /label=EYFP misc_feature 945..947 /label=stop misc_feature 209..948 /label=BBa_E0031 misc_feature 957..995 /label=B1006 stem_loop 966..987 /label=modified thr terminator polyA_site 988..995 /label=PolyA polyA_site 957..965 /label=PolyA terminator 957..995 /label=BBa_B1006 RBS 1004..1022 /label=Clontech RBS CDS 1023..1739 /label=ECFP misc_feature 1740..1742 /label=stop misc_feature 1004..1743 /label=BBa_E0021 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagga aagaggagaa attaagcatg gtgagcaagg 241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 421 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagcag cacgacttct 481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 661 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta ctagagaaaa 961 aaaaaccccg cccctgacag ggcggggttt ttttttacta gaggaaagag gagaaattaa 1021 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 1081 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 1141 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 1201 ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga 1261 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 1321 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 1381 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 1441 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga 1501 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 1561 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 1621 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 1681 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 1741 aat //