##gff-version 3
##sequence-region BBa_J70421 1 1744
BBa_J70421	.	engineered_region	209	948	.	+	0	ID=BBa_E0031;Name=BBa_E0031
BBa_J70421	.	ribosome_entry_site	210	228	.	+	0	ID=annotation353780;Name=Clontech RBS;Parent=BBa_E0031
BBa_J70421	.	stop_codon	945	947	.	+	0	ID=annotation353785;Name=stop;Parent=BBa_E0031
BBa_J70421	.	CDS	229	944	.	+	0	ID=annotation353784;Name=EYFP;Parent=BBa_E0031
BBa_J70421	.	engineered_region	1005	1744	.	+	0	ID=BBa_E0021;Name=BBa_E0021
BBa_J70421	.	stop_codon	1741	1743	.	+	0	ID=annotation353638;Name=stop;Parent=BBa_E0021
BBa_J70421	.	CDS	1024	1740	.	+	0	ID=annotation353637;Name=ECFP;Parent=BBa_E0021
BBa_J70421	.	ribosome_entry_site	1005	1023	.	+	0	ID=annotation353636;Name=Clontech RBS;Parent=BBa_E0021
BBa_J70421	.	terminator	957	996	.	+	0	ID=BBa_B1007;Name=BBa_B1007
BBa_J70421	.	engineered_region	957	996	.	+	0	ID=annotation1900955;Name=B1007;Parent=BBa_B1007
BBa_J70421	.	stem_loop	966	987	.	+	0	ID=annotation1900956;Name=modified thr;Parent=BBa_B1007
BBa_J70421	.	polyA_site	960	965	.	+	0	ID=annotation1900957;Name=PolyA;Parent=BBa_B1007
BBa_J70421	.	polyA_site	988	993	.	+	0	ID=annotation1900958;Name=PolyA;Parent=BBa_B1007
BBa_J70421	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J70421	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J70421	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J70421	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J70421	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J70421	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J70421	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J70421	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_J70421
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagaggaaagaggagaaattaagcatggtgagca
agggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgt
gtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtg
ccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagcagcacg
acttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagac
ccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggc
aacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggca
tcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccc
catcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgag
aagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaat
tactagagcgcaaaaaaccccgcccctgacagggcggggttttttcgctactagaggaaagaggagaaattaagcatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagc
agcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaaga
acggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agtaat