LOCUS       BBa_J70423              1967 bp    DNA     linear       22-Nov-2024
DEFINITION  R0010.J70399.E0040 or J70405.E0040
ACCESSION   BBa_J70423
VERSION     BBa_J70423.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..1241
                     /label=BBa_J70399
     CDS             1248..1967
                     /label=GFP protein
     CDS             1248..1967
                     /label=BBa_E0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat acatgaacat gcaatacgca aaccgcctct
      241 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      301 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      361 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      421 atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc
      481 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
      541 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
      601 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
      661 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
      721 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
      781 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
      841 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac
      901 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt
      961 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt
     1021 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
     1081 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
     1141 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1201 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagatg cgtaaaggag
     1261 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1321 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     1381 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
     1441 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
     1501 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     1561 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1621 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1681 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     1741 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1801 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1861 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1921 cagctgctgg gattacacat ggcatggatg aactatacaa ataataa
//