LOCUS       BBa_J70432              1960 bp    DNA     linear       30-Jun-2024
DEFINITION  R0010.J70428.E0040 or J70431.E0040
ACCESSION   BBa_J70432
VERSION     BBa_J70432.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..1234
                     /label=BBa_J70428
     CDS             1241..1960
                     /label=GFP protein
     CDS             1241..1960
                     /label=BBa_E0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa catgcaatac gcaaaccgcc tctccccgcg
      241 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      301 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      361 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag
      421 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      481 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      541 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      601 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      661 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      721 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      781 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      841 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      901 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      961 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     1021 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     1081 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
     1141 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1201 tcgttttatc tgttgtttgt cggtgaacgc tctctactag atgcgtaaag gagaagaact
     1261 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     1321 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1381 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
     1441 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
     1501 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
     1561 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     1621 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     1681 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     1741 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     1801 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     1861 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     1921 tgggattaca catggcatgg atgaactata caaataataa
//