LOCUS       BBa_J70472               948 bp    DNA     linear       15-Dec-2024
DEFINITION  R0010.B0033.E0030
ACCESSION   BBa_J70472
VERSION     BBa_J70472.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..219
                     /label=RBS-4\Weaker
     RBS             215..218
                     /label=RBS
     misc_feature    209..219
                     /label=BBa_B0033
     RBS             209..219
                     /label=BBa_B0033
     CDS             226..948
                     /label=BBa_E0030
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggact actagatggt gagcaagggc
      241 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      301 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      361 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      421 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc
      481 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      541 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      601 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      661 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      721 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      781 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
      841 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      901 accgccgccg ggatcactct cggcatggac gagctgtaca agtaataa
//