LOCUS BBa_J70501 4026 bp DNA linear 22-Dec-2024 DEFINITION Plasmid origin from pWV01 + Tet resistance gene for plasmid construction ACCESSION BBa_J70501 VERSION BBa_J70501.1 FEATURES Location/Qualifiers CDS 335..544 /label=orfB CDS 585..746 /label=orfC (CopG) CDS 813..1511 /label=repA CDS 1508..1657 /label=orfD misc_feature 50..181 /label=ir-I misc_feature 228..250 /label=ir-II misc_feature 334..382 /label=ir-III misc_feature 1656..1697 /label=ir-IV (terminator) promoter 522..550 /label=Promoter P1 misc_feature 456..466 /label=direct repeat 1 misc_feature 467..478 /label=direct repeat 2 misc_feature 479..490 /label=direct repeat 3 misc_feature 1..1747 /label=BBa_J70500 RBS 2094..2100 /label=RBS stem_loop 2074..2085 /label=term stem_loop 2028..2075 /label=stem_loop stem_loop 2009..2036 /label=stem_loop stem_loop 1997..2027 /label=stem_loop RBS 1992..1995 /label=rbs promoter 1823..1828 /label=-10 promoter 1800..1805 /label=-35 misc_feature 1835..1835 /label=+1 CDS 2005..2091 /label=leader peptide promoter 1756..2100 /label=BBa_J70025 CDS 2107..4026 /label=tetM gene CDS 2107..4026 /label=BBa_J70024 ORIGIN 1 cgatttttta ttaaaacgtc tcaaaatcgt ttctgagacg ttttagcgtt tatttcgttt 61 agttatcggc ataatcgtta aaacaggcgt tatcgtagcg taaaagccct tgagcgtagc 121 gtggctttgc agcgaagatg ttgtctgtta gattatgaaa gccgatgact gaatgaaata 181 ataagcgcag cgcccttcta tttcggttgg aggaggctca agggagtatg agggaatgaa 241 attccctcat gggtttgatt ttaaaaattg cttgcaattt tgccgagcgg tagcgctgga 301 aaatttttga aaaaaatttg gaatttggaa aaaaatgggg ggaaaggaag cgaattttgc 361 ttccgtacta cgacccccca ttaagtgccg agtgccaatt tttgtgccaa aaacgctcta 421 tcccaactgg ctcaagggtt taaggggttt ttcaatcgcc aacgaatcgc caacgttttc 481 gccaacgttt tttataaatc tatatttaag tagctttatt gttgttttta tgattacaaa 541 gtgatacact aactttataa aattatttga ttggagtttt ttaaatggtg atttcagaat 601 cgaaaaaaag agttatgatt tctctgacaa aagagcaaga taaaaaatta acagatatgg 661 cgaaacaaaa aggtttttca aaatctgcgg ttgcggcgtt agctatagaa gaatatgcaa 721 gaaaggaatc agaacaaaaa aaataagcga aagctcgcgt ttttagaagg atacgagttt 781 tcgctacttg tttttgataa ggtaattata tcatggctat taaaaatact aaagctagaa 841 attttggatt tttattatat cctgactcaa ttcctaatga ttggaaagaa aaattagaga 901 gtttgggcgt atctatggct gtcagtcctt tacacgatat ggacgaaaaa aaagataaag 961 atacatggaa tagtagtgat gttatacgaa atggaaagca ctataaaaaa ccacactatc 1021 acgttatata tattgcacga aatcctgtaa caatagaaag cgttaggaac aagattaagc 1081 gaaaattggg gaatagttca gttgctcatg ttgagatact tgattatatc aaaggttcat 1141 atgaatattt gactcatgaa tcaaaggacg ctattgctaa gaataaacat atatacgaca 1201 aaaaagatat tttgaacatt aatgattttg atattgaccg ctatataaca cttgatgaaa 1261 gccaaaaaag agaattgaag aatttacttt tagatatagt ggatgactat aatttggtaa 1321 atacaaaaga tttaatggct tttattcgcc ttaggggagc ggagtttgga attttaaata 1381 cgaatgatgt aaaagatatt gtttcaacaa actctagcgc ctttagatta tggtttgagg 1441 gcaattatca gtgtggatat agagcaagtt atgcaaaggt tcttgatgct gaaacggggg 1501 aaataaaatg acaaacaaag aaaaagagtt atttgctgaa aatgaggaat taaaaaaaga 1561 aattaaggac ttaaaagagc gtattgaaag atacagagaa atggaagttg aattaagtac 1621 aacaatagat ttattgagag gagggattat tgaataaata aaagccccct gacgaaagtc 1681 gaagggggtt tttattttgg tttgatgttg cgattaatag caatacaatt gcaataaaca 1741 aaatgattac tagagggcca gtctacatgt actctttttg ataaaaaatt ggagattcct 1801 ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag ttaataaata 1861 tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat aattagatgt 1921 ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta tcatgtgatt 1981 ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca taaaaatccc 2041 agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg aatggaggaa 2101 tactagatga aaatcataaa tatcggtgta ttagctcacg ttgatgcagg aaaaacaaca 2161 ttaactgaat cacttttata taactctggt gcaattactg aacttggttc agtagataaa 2221 ggtactactc gtactgataa tacattatta gaacgtcaac gtggaatcac aattcaaaca 2281 ggtatcacat cttttcaatg ggaaaataca aaagtaaata ttatagatac acctggacac 2341 atggatttcc ttgcagaagt ataccgtagt ctttcagtat tagatggtgc tattttactt 2401 atcagcgcta aagatggagt tcaagctcaa actcgtatct tatttcacgc attacgtaaa 2461 atgggtattc caacaatttt ctttataaac aaaattgacc aaaacggaat tgatttaagt 2521 acagtttatc aagatatcaa agaaaaactt tctgctgaaa tcgttattaa acaaaaagtt 2581 gaattatacc caaacgtttg cgtaacaaat tttactgaat cagaacaatg ggatacagtt 2641 atagaaggta atgatgattt attagaaaaa tacatgtcag gtaaatcatt agaagcatta 2701 gaattagaac aagaagaaag tattcgtttc caaaactgtt ctttattccc tttataccat 2761 ggaagcgcta aaagtaacat aggtattgat aacttaattg aagttattac taacaaattt 2821 tactcttcaa ctcatcgtgg tccttctgaa ttatgcggta acgttttcaa aattgaatat 2881 acaaaaaaac gtcaacgttt agcttatata cgtctttata gtggtgtttt acatttacgt 2941 gatagtgttc gtgttagtga aaaagaaaag attaaagtta cagaaatgta tacttctatt 3001 aacggtgaat tatgcaaaat tgaccgtgca tattcaggtg aaattgtaat tttacaaaac 3061 gaatttctta aacttaatag tgtacttggt gacacaaaac ttttaccaca acgtaagaaa 3121 attgaaaatc cacacccatt acttcaaaca acagtagaac caagcaaacc tgaacaacgt 3181 gaaatgcttt tagatgctct tttagaaatt agtgactctg acccactttt acgttactat 3241 gtagattcta ctactcatga aattattctt tctttccttg gtaaagttca aatggaagtt 3301 atttctgcat tattacaaga aaaatatcat gttgaaatcg aattaaaaga acctactgta 3361 atttatatgg aacgtccatt aaaaaatgct gaatatacaa ttcatattga agttccacca 3421 aatccatttt gggcttctat tggtctttct gtttctccac ttccacttgg tagcggaatg 3481 caatatgaaa gtagcgtaag tttaggttat cttaatcaaa gtttccaaaa cgcagttatg 3541 gaaggtattc gttacggttg cgaacaaggt ttatacggtt ggaatgttac agactgcaaa 3601 atctgtttta agtatggact ttactattca cctgtatcaa cacctgctga ctttcgtatg 3661 cttgcaccaa ttgttttaga acaagtttta aagaaagctg gaactgaact tttagaacca 3721 tacctttctt ttaaaatcta tgcaccacaa gaatacttaa gtcgtgctta taacgatgca 3781 cctaaatact gtgctaatat tgttgatact caattaaaga acaacgaagt aattttaagc 3841 ggagaaattc ctgcacgttg tattcaagaa tatcgtagtg atttaacatt tttcactaat 3901 ggacgttctg tttgcttaac tgaattaaaa ggttatcatg ttactactgg tgaacctgta 3961 tgccaaccac gtcgtcctaa tagtcgtatt gataaagttc gttatatgtt caacaaaatc 4021 acataa //