LOCUS BBa_J70502 4026 bp DNA linear 22-Dec-2024 DEFINITION Plasmid origin from pWV01 + Tet resistance gene for plasmid construction ACCESSION BBa_J70502 VERSION BBa_J70502.1 FEATURES Location/Qualifiers RBS 339..345 /label=RBS stem_loop 319..330 /label=term stem_loop 273..320 /label=stem_loop stem_loop 254..281 /label=stem_loop stem_loop 242..272 /label=stem_loop RBS 237..240 /label=rbs promoter 68..73 /label=-10 promoter 45..50 /label=-35 misc_feature 80..80 /label=+1 CDS 250..336 /label=leader peptide promoter 1..345 /label=BBa_J70025 CDS 352..2271 /label=tetM gene CDS 352..2271 /label=BBa_J70024 CDS 2614..2823 /label=orfB CDS 2864..3025 /label=orfC (CopG) CDS 3092..3790 /label=repA CDS 3787..3936 /label=orfD misc_feature 2329..2460 /label=ir-I misc_feature 2507..2529 /label=ir-II misc_feature 2613..2661 /label=ir-III misc_feature 3935..3976 /label=ir-IV (terminator) promoter 2801..2829 /label=Promoter P1 misc_feature 2735..2745 /label=direct repeat 1 misc_feature 2746..2757 /label=direct repeat 2 misc_feature 2758..2769 /label=direct repeat 3 misc_feature 2280..4026 /label=BBa_J70500 ORIGIN 1 ggccagtcta catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct 61 tacgtgctat tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc 121 ttaaataaac tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg 181 cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag 241 aatatctgta tgctttgtat acctatggtt atgcataaaa atcccagtga taaaagtatt 301 tatcactggg atttttatgc ccttttgggt ttttgaatgg aggaatacta gatgaaaatc 361 ataaatatcg gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt 421 ttatataact ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact 481 gataatacat tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt 541 caatgggaaa atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca 601 gaagtatacc gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat 661 ggagttcaag ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca 721 attttcttta taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat 781 atcaaagaaa aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac 841 gtttgcgtaa caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat 901 gatttattag aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa 961 gaaagtattc gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt 1021 aacataggta ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat 1081 cgtggtcctt ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa 1141 cgtttagctt atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt 1201 agtgaaaaag aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc 1261 aaaattgacc gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt 1321 aatagtgtac ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac 1381 ccattacttc aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat 1441 gctcttttag aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact 1501 catgaaatta ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta 1561 caagaaaaat atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt 1621 ccattaaaaa atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct 1681 tctattggtc tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc 1741 gtaagtttag gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac 1801 ggttgcgaac aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat 1861 ggactttact attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt 1921 ttagaacaag ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa 1981 atctatgcac cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct 2041 aatattgttg atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca 2101 cgttgtattc aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc 2161 ttaactgaat taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt 2221 cctaatagtc gtattgataa agttcgttat atgttcaaca aaatcacata atactagagc 2281 gattttttat taaaacgtct caaaatcgtt tctgagacgt tttagcgttt atttcgttta 2341 gttatcggca taatcgttaa aacaggcgtt atcgtagcgt aaaagccctt gagcgtagcg 2401 tggctttgca gcgaagatgt tgtctgttag attatgaaag ccgatgactg aatgaaataa 2461 taagcgcagc gcccttctat ttcggttgga ggaggctcaa gggagtatga gggaatgaaa 2521 ttccctcatg ggtttgattt taaaaattgc ttgcaatttt gccgagcggt agcgctggaa 2581 aatttttgaa aaaaatttgg aatttggaaa aaaatggggg gaaaggaagc gaattttgct 2641 tccgtactac gaccccccat taagtgccga gtgccaattt ttgtgccaaa aacgctctat 2701 cccaactggc tcaagggttt aaggggtttt tcaatcgcca acgaatcgcc aacgttttcg 2761 ccaacgtttt ttataaatct atatttaagt agctttattg ttgtttttat gattacaaag 2821 tgatacacta actttataaa attatttgat tggagttttt taaatggtga tttcagaatc 2881 gaaaaaaaga gttatgattt ctctgacaaa agagcaagat aaaaaattaa cagatatggc 2941 gaaacaaaaa ggtttttcaa aatctgcggt tgcggcgtta gctatagaag aatatgcaag 3001 aaaggaatca gaacaaaaaa aataagcgaa agctcgcgtt tttagaagga tacgagtttt 3061 cgctacttgt ttttgataag gtaattatat catggctatt aaaaatacta aagctagaaa 3121 ttttggattt ttattatatc ctgactcaat tcctaatgat tggaaagaaa aattagagag 3181 tttgggcgta tctatggctg tcagtccttt acacgatatg gacgaaaaaa aagataaaga 3241 tacatggaat agtagtgatg ttatacgaaa tggaaagcac tataaaaaac cacactatca 3301 cgttatatat attgcacgaa atcctgtaac aatagaaagc gttaggaaca agattaagcg 3361 aaaattgggg aatagttcag ttgctcatgt tgagatactt gattatatca aaggttcata 3421 tgaatatttg actcatgaat caaaggacgc tattgctaag aataaacata tatacgacaa 3481 aaaagatatt ttgaacatta atgattttga tattgaccgc tatataacac ttgatgaaag 3541 ccaaaaaaga gaattgaaga atttactttt agatatagtg gatgactata atttggtaaa 3601 tacaaaagat ttaatggctt ttattcgcct taggggagcg gagtttggaa ttttaaatac 3661 gaatgatgta aaagatattg tttcaacaaa ctctagcgcc tttagattat ggtttgaggg 3721 caattatcag tgtggatata gagcaagtta tgcaaaggtt cttgatgctg aaacggggga 3781 aataaaatga caaacaaaga aaaagagtta tttgctgaaa atgaggaatt aaaaaaagaa 3841 attaaggact taaaagagcg tattgaaaga tacagagaaa tggaagttga attaagtaca 3901 acaatagatt tattgagagg agggattatt gaataaataa aagccccctg acgaaagtcg 3961 aagggggttt ttattttggt ttgatgttgc gattaatagc aatacaattg caataaacaa 4021 aatgat //