BBa_J70024 1 BBa_J70024 TetM gene, recoded compromise E. coli & Mesoplasma florum 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding. The tetM antibiotic resistance gene synthesized by Geneart from a protein sequence derived from the Ureaplasma urealyticum tetM gene. Synthesized with a compromise codon usage table for expression in both E. coli and Mesoplasma florum. Highly active in both E. coli and Mesoplasma florum. false false _41_48_1_ 0 6 48 Not in stock true Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species. false Tom Knight annotation1982961 1 tetM gene range1982961 1 1 1920 BBa_J70025 1 BBa_J70025 Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid. Upstream promoter for tetM gene; contains a short peptide leader, regulatory sequences which are poorly understood. Use in combination with J70024. false false _41_48_1_ 0 6 48 Not in stock true Alignment of the promoter with the coding region, preservation of the RBS sites. false Tom Knight annotation1999385 1 stem_loop range1999385 1 254 281 annotation1999382 1 RBS range1999382 1 339 345 annotation1999389 1 -35 range1999389 1 45 50 annotation1999391 1 leader peptide range1999391 1 250 336 annotation1999383 1 term range1999383 1 319 330 annotation1999388 1 -10 range1999388 1 68 73 annotation1999386 1 stem_loop range1999386 1 242 272 annotation1999390 1 +1 range1999390 1 80 80 annotation1999387 1 rbs range1999387 1 237 240 annotation1999384 1 stem_loop range1999384 1 273 320 BBa_J70513 1 BBa_J70513 Tet resistance + Mesoplasma origin fragment 2009-10-03T11:00:00Z 2015-05-08T01:08:23Z Assembly. Tet resistance + Mesoplasma origin fragment false false _41_48_1_ 0 6 48 Not in stock false None. false Tom Knight component2028121 1 BBa_J70510 component2028118 1 BBa_J70025 component2028120 1 BBa_J70024 annotation2028120 1 BBa_J70024 range2028120 1 352 2271 annotation2028118 1 BBa_J70025 range2028118 1 1 345 annotation2028121 1 BBa_J70510 range2028121 1 2280 2652 BBa_J70510 1 BBa_J70510 Mesoplasma florum origin of replication 2009-10-02T11:00:00Z 2015-05-08T01:08:23Z PCR from genomic DNA Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F3 to OR-R4 GTTTCTTC T TCTAGA G gaatttaaaaaaatagttgagtc,OR-F3 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A cctgtaatgctgggttaac,OR-R4 false false _41_48_1_ 0 6 48 Not in stock false Primer F3 mutates out an EcoRI site. false Tom Knight BBa_J70510_sequence 1 gaatttaaaaaaatagttgagtctttaaaaaacgaaataataggaaaatagatgtaaattcgcatctattttttttgtgtggaaaagaaattaaacttgttgaataaaaagtcatttaaacctttatttaccgttgttttatgagtgttttccacatatcaactgcattataaatattacttatataaagaataatataatatataaaagcatgcgtatttcttttgtttttattttataaatatgagataatatatattgtaaataagaggtgttatatgagattaagtataaataaatcagttctattaaatgaattaacaaaggcaagtaaattaattgaacttaaaaatgttaacccagcattacaagg BBa_J70024_sequence 1 atgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa BBa_J70513_sequence 1 ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaatactagatgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataatactagaggaatttaaaaaaatagttgagtctttaaaaaacgaaataataggaaaatagatgtaaattcgcatctattttttttgtgtggaaaagaaattaaacttgttgaataaaaagtcatttaaacctttatttaccgttgttttatgagtgttttccacatatcaactgcattataaatattacttatataaagaataatataatatataaaagcatgcgtatttcttttgtttttattttataaatatgagataatatatattgtaaataagaggtgttatatgagattaagtataaataaatcagttctattaaatgaattaacaaaggcaagtaaattaattgaacttaaaaatgttaacccagcattacaagg BBa_J70025_sequence 1 ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z