BBa_J70491 1 BBa_J70491 gfp RBS, {0,5;15,10} family member - level 10000 (master sequence) 2009-08-31T11:00:00Z 2015-05-08T01:08:23Z designed These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;15,10})-GFP to (Promoter X)-(RBS level Y)-GFP. Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.) (In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.) Form of insert: {promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)} {promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)} Forward oligo: g {rbs master sequence} atg c gtaaa Reverse oligo: Reverse complement of (ctagag {rbs master sequence} atg c) false false _41_ 0 1201 41 Not in stock false none false Julie Norville BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_J70517 1 BBa_J70517 10000gfp(J70491).E0040 no scar 2009-12-08T12:00:00Z 2015-05-08T01:08:23Z BioScaffold Intermediate 10000gfp(J70491).E0040 no scar false true _41_ 0 1201 41 Not in stock false BioScaffold Intermediate false Julie Norville component2064522 1 BBa_J70491 component2064524 1 BBa_E0040 annotation2064522 1 BBa_J70491 range2064522 1 1 33 annotation2064524 1 BBa_E0040 range2064524 1 34 753 BBa_J70491_sequence 1 tgtcgcggatactgatccataaaggccggggtt BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J70517_sequence 1 tgtcgcggatactgatccataaaggccggggttatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z