LOCUS       BBa_J70519               962 bp    DNA     linear       22-Nov-2024
DEFINITION  formed by cutting J70423 with PsrI and adding annealed J70483 and 
            J70484
ACCESSION   BBa_J70519
VERSION     BBa_J70519.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..242
                     /label=BBa_J70482
     CDS             243..962
                     /label=GFP protein
     CDS             243..962
                     /label=BBa_E0040
     misc_feature    209..962
                     /label=BBa_J70514
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagct aaataggagg ctgggagttc aacgaaaccc
      241 ctatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      301 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      361 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      421 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      481 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      541 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      601 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      661 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      721 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      781 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      841 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      901 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
      961 aa
//