LOCUS       BBa_J70545               945 bp    DNA     linear       15-Dec-2024
DEFINITION  R0010.B0033.E0040
ACCESSION   BBa_J70545
VERSION     BBa_J70545.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..219
                     /label=RBS-4\Weaker
     RBS             215..218
                     /label=RBS
     misc_feature    209..219
                     /label=BBa_B0033
     RBS             209..219
                     /label=BBa_B0033
     CDS             226..945
                     /label=GFP protein
     CDS             226..945
                     /label=BBa_E0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggact actagatgcg taaaggagaa
      241 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      301 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      361 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
      421 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      481 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      541 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      601 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      661 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      721 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      781 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      841 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      901 gctgctggga ttacacatgg catggatgaa ctatacaaat aataa
//