LOCUS BBa_J70575 1049 bp DNA linear 22-Dec-2024 DEFINITION {15,18;13,10} BsaXI BioScaffold v 5.0, no BsaXI, V169I in E0040 ACCESSION BBa_J70575 VERSION BBa_J70575.1 FEATURES Location/Qualifiers misc_feature 1..4 /label=BsaXI partial site (only occurs when follows scar) misc_feature 795..805 /label=BsaXI site misc_feature 1040..1049 /label=BsaXI partial site (occurs with suffix or scar) misc_feature 506..506 /label=mutation from J70568: G506A (DNA), V169I (protein) promoter 1..170 /label=R0010(-naturally occurring internal BsaXI site) RBS 178..190 /label=B0034 CDS 197..877 /label=E1010 misc_feature 878..902 /label=Junk from J04450 misc_feature 12..14 /label=BsaXI potential cut site misc_feature 783..785 /label=BsaXI cut site misc_feature 813..815 /label=BsaXI cut site misc_feature 1030..1032 /label=BsaXI cut site misc_feature 911..990 /label=B0010 misc_feature 999..1039 /label=B0012 ORIGIN 1 ctccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 61 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa 181 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa 241 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg 301 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc 361 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca 421 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg 481 tgttatgaac ttcgaagacg gtggtattgt taccgttacc caggactcct ccctgcaaga 541 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt 601 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg 661 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc 721 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac 781 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga 841 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc 901 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 961 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1021 gggcctttct gcgtttatag gaggacagg //