##gff-version 3
##sequence-region BBa_J70577 1 1969
BBa_J70577	.	engineered_region	1	912	.	+	0	ID=BBa_J70570;Name=BBa_J70570
BBa_J70577	.	promoter	1	166	.	+	0	ID=annotation2067734;Name=BsaXI removed, R0010;Parent=BBa_J70570
BBa_J70577	.	CDS	194	912	.	+	0	ID=annotation2067736;Name=E0040;Parent=BBa_J70570
BBa_J70577	.	ribosome_entry_site	175	186	.	+	0	ID=annotation2067735;Name=B0034;Parent=BBa_J70570
BBa_J70577	.	sequence_feature	921	1969	.	+	0	ID=BBa_J70575;Name=BBa_J70575
BBa_J70577	.	sequence_feature	1950	1952	.	+	0	ID=annotation2069026;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70577	.	engineered_region	1831	1910	.	+	0	ID=annotation2069027;Name=B0010;Parent=BBa_J70575
BBa_J70577	.	CDS	1117	1797	.	+	0	ID=annotation2069021;Name=E1010;Parent=BBa_J70575
BBa_J70577	.	engineered_region	1919	1959	.	+	0	ID=annotation2069028;Name=B0012;Parent=BBa_J70575
BBa_J70577	.	ribosome_entry_site	1098	1110	.	+	0	ID=annotation2069020;Name=B0034;Parent=BBa_J70575
BBa_J70577	.	sequence_feature	932	934	.	+	0	ID=annotation2069023;Name=BsaXI potential cut site;Parent=BBa_J70575
BBa_J70577	.	sequence_feature	1960	1969	.	+	0	ID=annotation2069017;Name=BsaXI partial site (occurs with suffix or scar);Parent=BBa_J70575
BBa_J70577	.	sequence_feature	1733	1735	.	+	0	ID=annotation2069025;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70577	.	promoter	921	1090	.	+	0	ID=annotation2069019;Name=R0010(-naturally occurring internal BsaXI site);Parent=BBa_J70575
BBa_J70577	.	sequence_feature	1715	1725	.	+	0	ID=annotation2069016;Name=BsaXI site;Parent=BBa_J70575
BBa_J70577	.	sequence_feature	921	924	.	+	0	ID=annotation2069015;Name=BsaXI partial site (only occurs when follows scar);Parent=BBa_J70575
BBa_J70577	.	sequence_alteration	1426	1426	.	+	0	ID=annotation2069018;Name=mutation from J70568: G506A (DNA), V169I (protein);Parent=BBa_J70575
BBa_J70577	.	sequence_feature	1703	1705	.	+	0	ID=annotation2069024;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70577	.	sequence_feature	1798	1822	.	+	0	ID=annotation2069022;Name=Junk from J04450;Parent=BBa_J70575
>BBa_J70577
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag
ctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattctt
gttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaac
ttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgt
tcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacag
gaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtta
atagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactc
acacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagat
ggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatt
acctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagc
tgctgggattacacatggcatggatgaactatacaaataataatactagagctccgattcattaatgcagctggcacga
caggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggct
ttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagagga
gaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaac
ggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttacca
aaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacaccc
ggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggt
ggtattgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaact
tcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacgg
tgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctac
atggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagact
acaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtag
atcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggaggacagg