LOCUS       BBa_J70589              1949 bp    DNA     linear       06-Dec-2025
DEFINITION  N-terminal protein tag addition test part J70576:S04431(J70575:E004
            0)
ACCESSION   BBa_J70589
VERSION     BBa_J70589.1  
FEATURES             Location/Qualifiers
     promoter        1..166
                     /label=BBa_J70576
     misc_feature    175..178
                     /label=BsaXI partial site (only occurs when follows scar)
     misc_feature    969..979
                     /label=BsaXI site
     misc_feature    1214..1223
                     /label=BsaXI partial site (occurs with suffix or scar)
     misc_feature    680..680
                     /label=mutation from J70568: G506A (DNA), V169I (protein)
     promoter        175..344
                     /label=R0010(-naturally occurring internal BsaXI site)
     RBS             352..364
                     /label=B0034
     CDS             371..1051
                     /label=E1010
     misc_feature    1052..1076
                     /label=Junk from J04450
     misc_feature    186..188
                     /label=BsaXI potential cut site
     misc_feature    957..959
                     /label=BsaXI cut site
     misc_feature    987..989
                     /label=BsaXI cut site
     misc_feature    1204..1206
                     /label=BsaXI cut site
     misc_feature    1085..1164
                     /label=B0010
     misc_feature    1173..1213
                     /label=B0012
     misc_feature    175..1223
                     /label=BBa_J70575
     CDS             1230..1949
                     /label=GFP protein
     CDS             1230..1949
                     /label=BBa_E0040
ORIGIN
        1 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
       61 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      121 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagctccga
      181 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      241 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      301 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga
      361 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      421 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      481 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      541 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      601 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
      661 gaacttcgaa gacggtggta ttgttaccgt tacccaggac tcctccctgc aagacggtga
      721 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
      781 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
      841 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
      901 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
      961 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1021 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact
     1081 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1141 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1201 ttctgcgttt ataggaggac aggtactaga tgcgtaaagg agaagaactt ttcactggag
     1261 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1321 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1381 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1441 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1501 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1561 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1621 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1681 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     1741 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1801 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     1861 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     1921 atggcatgga tgaactatac aaataataa
//