##gff-version 3
##sequence-region BBa_J70589 1 1949
BBa_J70589	.	CDS	1230	1949	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J70589	.	CDS	1230	1949	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J70589	.	sequence_feature	175	1223	.	+	0	ID=BBa_J70575;Name=BBa_J70575
BBa_J70589	.	sequence_feature	1204	1206	.	+	0	ID=annotation2069026;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70589	.	engineered_region	1085	1164	.	+	0	ID=annotation2069027;Name=B0010;Parent=BBa_J70575
BBa_J70589	.	CDS	371	1051	.	+	0	ID=annotation2069021;Name=E1010;Parent=BBa_J70575
BBa_J70589	.	engineered_region	1173	1213	.	+	0	ID=annotation2069028;Name=B0012;Parent=BBa_J70575
BBa_J70589	.	ribosome_entry_site	352	364	.	+	0	ID=annotation2069020;Name=B0034;Parent=BBa_J70575
BBa_J70589	.	sequence_feature	186	188	.	+	0	ID=annotation2069023;Name=BsaXI potential cut site;Parent=BBa_J70575
BBa_J70589	.	sequence_feature	1214	1223	.	+	0	ID=annotation2069017;Name=BsaXI partial site (occurs with suffix or scar);Parent=BBa_J70575
BBa_J70589	.	sequence_feature	987	989	.	+	0	ID=annotation2069025;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70589	.	promoter	175	344	.	+	0	ID=annotation2069019;Name=R0010(-naturally occurring internal BsaXI site);Parent=BBa_J70575
BBa_J70589	.	sequence_feature	969	979	.	+	0	ID=annotation2069016;Name=BsaXI site;Parent=BBa_J70575
BBa_J70589	.	sequence_feature	175	178	.	+	0	ID=annotation2069015;Name=BsaXI partial site (only occurs when follows scar);Parent=BBa_J70575
BBa_J70589	.	sequence_alteration	680	680	.	+	0	ID=annotation2069018;Name=mutation from J70568: G506A (DNA), V169I (protein);Parent=BBa_J70575
BBa_J70589	.	sequence_feature	957	959	.	+	0	ID=annotation2069024;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70589	.	sequence_feature	1052	1076	.	+	0	ID=annotation2069022;Name=Junk from J04450;Parent=BBa_J70575
BBa_J70589	.	promoter	1	166	.	+	0	ID=BBa_J70576;Name=BBa_J70576
>BBa_J70589
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag
ctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacatactagagctccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgca
acgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgg
aattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatca
aagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaagg
tcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctg
tccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcc
cggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtattgttaccgttacccaggactcctccctgca
agacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacc
atgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaac
tgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctta
caaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggt
cgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttataggaggacaggtactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa