BBa_J70554 1 BBa_J70554 Split GFP C-terminal domain 2010-02-17T12:00:00Z 2015-05-08T01:08:24Z Pcr'd from J04430 Forward primer (J70554-f): ttcgaattcgcggccgcttctagATGaagaatggaatcaaagttaacttcaaaattag Reverse primer (J70554-r): gtttcttcctgcagcggccgctactagtataaacgcagaaaggcccacccgaa From Lynne Regan design and J04430 false false _41_ 0 1201 41 Not in stock false added an ATG at the start of the sequence false Julie Norville BBa_J70575 1 BBa_J70575 {15,18;13,10} BsaXI BioScaffold v 5.0, no BsaXI, V169I in E0040 2010-05-08T11:00:00Z 2015-05-08T01:08:24Z designed (pcr'd from J04450) J70568f: gtttcttcgaattcgcggccgcttctagagctccgattcattaatgcagctggcacgac J70568r: gtttcttcctgcagcggccgctactagtacctgtcctcctataaacgcagaaag removed the BsaXI site from the R0010 portion of the part a mutation in version J70568 left the red color of E0040, but otherwise was correct, so that is the difference between this and J70568 false false _41_ 0 1201 41 Not in stock false this removes BsaXI site from R0010 and incorporates a PCR mutation that occurred when trying to amplify J70568 false Julie Norville annotation2069024 1 BsaXI cut site range2069024 1 783 785 annotation2069026 1 BsaXI cut site range2069026 1 1030 1032 annotation2069027 1 B0010 range2069027 1 911 990 annotation2069021 1 E1010 range2069021 1 197 877 annotation2069017 1 BsaXI partial site (occurs with suffix or scar) range2069017 1 1040 1049 annotation2069020 1 B0034 range2069020 1 178 190 annotation2069028 1 B0012 range2069028 1 999 1039 annotation2069025 1 BsaXI cut site range2069025 1 813 815 annotation2069022 1 Junk from J04450 range2069022 1 878 902 annotation2069016 1 BsaXI site range2069016 1 795 805 annotation2069019 1 R0010(-naturally occurring internal BsaXI site) range2069019 1 1 170 annotation2069015 1 BsaXI partial site (only occurs when follows scar) range2069015 1 1 4 annotation2069023 1 BsaXI potential cut site range2069023 1 12 14 annotation2069018 1 mutation from J70568: G506A (DNA), V169I (protein) range2069018 1 506 506 BBa_J70590 1 BBa_J70590 Protein fusion test part S04432(J70569:J70575):J70554 2010-06-15T11:00:00Z 2015-05-08T01:08:24Z BioBrick assembly, to add GSGSGS fusion add following annealed oligos: J70556-fi J70556-ric To add a dead fusion (i.e. a stop codon), add the following annealed oligo nucleotides: deadfusion J70556d-fi deadfusion J70556d-ri S04432 is in pSB1AC3, J70554 is in pSB1AC3; thus J70590 will probably be in pSB1AK3 the goal is to see if the BioScaffold can be replaced with an internal GSGSGS fusion, also will replace with a dead fusion false false _41_ 0 1201 41 Not in stock false none false Julie Norville component2070929 1 BBa_J70569 component2070945 1 BBa_J70554 component2070944 1 BBa_J70575 annotation2070929 1 BBa_J70569 range2070929 1 1 669 annotation2070945 1 BBa_J70554 range2070945 1 1733 2119 annotation2070944 1 BBa_J70575 range2070944 1 678 1726 BBa_J70569 1 BBa_J70569 Expressed N-terminal split GFP 2010-04-10T11:00:00Z 2015-05-08T01:08:24Z Pcr'd from J04430 with BsaXI site removed. J70569f: gtttcttcgaattcgcggccgcttctagaggattcattaatgcagctggcacgac J70569r: tcttcctgcagcggccgctactagtattattattgtttgtctgccatgatgtatacattg Lynne Reagan version of split GFP n-terminus, derived from J04430. false false _41_ 0 1201 41 Not in stock false Remove BsaXI site. false Julie Norville annotation2067732 1 R0010, BsaXI site removed range2067732 1 1 186 annotation2067733 1 N terminal E0040 range2067733 1 193 669 BBa_J70590_sequence 1 gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaataataatactagagctccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtattgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggaggacaggtactagatgaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta BBa_J70554_sequence 1 atgaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta BBa_J70569_sequence 1 gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaataataa BBa_J70575_sequence 1 ctccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtattgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggaggacagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z