##gff-version 3
##sequence-region BBa_J70590 1 2119
BBa_J70590	.	polypeptide_domain	1733	2119	.	+	0	ID=BBa_J70554;Name=BBa_J70554
BBa_J70590	.	sequence_feature	678	1726	.	+	0	ID=BBa_J70575;Name=BBa_J70575
BBa_J70590	.	engineered_region	1676	1716	.	+	0	ID=annotation2069028;Name=B0012;Parent=BBa_J70575
BBa_J70590	.	promoter	678	847	.	+	0	ID=annotation2069019;Name=R0010(-naturally occurring internal BsaXI site);Parent=BBa_J70575
BBa_J70590	.	sequence_feature	1707	1709	.	+	0	ID=annotation2069026;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70590	.	CDS	874	1554	.	+	0	ID=annotation2069021;Name=E1010;Parent=BBa_J70575
BBa_J70590	.	sequence_feature	678	681	.	+	0	ID=annotation2069015;Name=BsaXI partial site (only occurs when follows scar);Parent=BBa_J70575
BBa_J70590	.	sequence_feature	1460	1462	.	+	0	ID=annotation2069024;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70590	.	sequence_feature	1490	1492	.	+	0	ID=annotation2069025;Name=BsaXI cut site;Parent=BBa_J70575
BBa_J70590	.	sequence_feature	1717	1726	.	+	0	ID=annotation2069017;Name=BsaXI partial site (occurs with suffix or scar);Parent=BBa_J70575
BBa_J70590	.	sequence_feature	689	691	.	+	0	ID=annotation2069023;Name=BsaXI potential cut site;Parent=BBa_J70575
BBa_J70590	.	sequence_alteration	1183	1183	.	+	0	ID=annotation2069018;Name=mutation from J70568: G506A (DNA), V169I (protein);Parent=BBa_J70575
BBa_J70590	.	ribosome_entry_site	855	867	.	+	0	ID=annotation2069020;Name=B0034;Parent=BBa_J70575
BBa_J70590	.	engineered_region	1588	1667	.	+	0	ID=annotation2069027;Name=B0010;Parent=BBa_J70575
BBa_J70590	.	sequence_feature	1472	1482	.	+	0	ID=annotation2069016;Name=BsaXI site;Parent=BBa_J70575
BBa_J70590	.	sequence_feature	1555	1579	.	+	0	ID=annotation2069022;Name=Junk from J04450;Parent=BBa_J70575
BBa_J70590	.	CDS	1	669	.	+	0	ID=BBa_J70569;Name=BBa_J70569
BBa_J70590	.	CDS	193	669	.	+	0	ID=annotation2067733;Name=N terminal E0040;Parent=BBa_J70569
BBa_J70590	.	sequence_feature	1	186	.	+	0	ID=annotation2067732;Name=R0010, BsaXI site removed;Parent=BBa_J70569
>BBa_J70590
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag
ctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattctt
gttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaac
ttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgt
tcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacag
gaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtta
atagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactc
acacaatgtatacatcatggcagacaaacaataataatactagagctccgattcattaatgcagctggcacgacaggtt
tcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacac
tttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaata
ctagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcac
gagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtg
gtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctga
catcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtatt
gttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgt
ccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctct
gaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggct
aaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacacca
tcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgct
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggaggacaggtactagatgaag
aatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaa
atactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcc
caacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatac
aaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta