BBa_J70593 1 BBa_J70593 RFC12 ATG Head Domain 2010-06-17T11:00:00Z 2015-05-08T01:08:25Z Common knowledge. A RFC12 compatible part that codes for a single amino acid, methionine. This is a head domain meant to be assembled directly in front of internal domains. Please remember that modification of the first few codons can drastically affect translation efficiency. If you are worried about modification of the N-terminal design your own head domain with the first two or three codons included. false false _41_ 0 6384 41 Not in stock false Made with synthetic 5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F 5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. false Joseph Lynch annotation2071256 1 start range2071256 1 1 3 BBa_J70593_sequence 1 atg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z