BBa_J70604 1 BBa_J70604 J70589 cut with BsaXI and with rbs, 6his inserts (J70559-f1i,f2i,r2i,r2i) added 2010-06-27T11:00:00Z 2015-05-08T01:08:25Z Look at part J70575 This tests adding a protein fusion to the n-terminus of a protein using J70589 as a test case. false true _41_ 0 1201 41 Not in stock false Created by cutting part J70589 with BsaXI and adding rbs, 6 his-glycine serine inserts (legacy from prior design) forward1 (J70559-f1i): TTCACACATACTAGAGAAAGAGGAGAAATACTAGATGCATCATC forward2 (J70559-f2i): ATCATCATCACAGCAGCGGCcgta reverse1 (J70559-r1i): CTCTAGTATGTGTGAAatt reverse2 (J70559-r2i): gGCCGCTGCTGTGATGATGATGATGATGCATCTAGTATTTCTCCTCTTT false Julie Norville BBa_J70604_sequence 1 gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcatcatcatcatcatcacagcagcggccgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z