BBa_J70591 1 BBa_J70591 RFC12 Elowitz RBS 2010-06-17T11:00:00Z 2015-05-08T01:08:24Z Part BBa_B0034 Released HQ 2013 This is a RFC12 compatible version of BBa_B0034. It is a purine rich RBS with efficiency 1 (definition). false false _41_ 0 6384 41 In stock false Designed with synthetic oligos: RBS-F: 5' AATTC GCGGCGC T ACTAGT AAAGAGGAGAAA GCTAGC A GCGGCCG CTGCA 3' RBS-R 5' GCGGCCGCTGCTAGC TTTCTCCTCTTT ACTAGTAGCGCCGCG 3' false Joseph Lynch annotation2071168 1 rbs range2071168 1 5 8 BBa_J70592 1 BBa_J70592 RFC12 Constitutive Promoter 2010-06-17T11:00:00Z 2015-05-08T01:08:25Z BBa_J23100 This is a RFC12 compatible version of part BBa_J23100. It is a constitutive promoter isolated from a small combinatorial library. false false _41_ 0 6384 41 Not in stock false Designed with synthetic oligos: 5' AATTC GCGGCGC T ACTAGT TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC GCTAGC A GCGGCCG CTGCA 3', Prom-F 5' GCGGCCGCTGCTAGC GCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAA ACTAGTAGCGCCGCG 3', Prom-R Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. false Joseph Lynch annotation2076867 1 -10 range2076867 1 23 28 annotation2076866 1 -35 range2076866 1 1 6 BBa_J70608 1 BBa_J70608 RFC12 Constitutive Promoter and a RBS 2010-07-12T11:00:00Z 2015-05-08T01:08:25Z BBa_J70592 + BBa_J70591 This is a RFC12 assembly of BBa_J70592 and BBa_J70591 so that the end user does not need to assemble the RBS into the reaction every time. If you need more control over either the promoter or the RBS do not use this part. false false _41_ 0 6384 41 Not in stock false Assembled using standard 3a assembly method. false Joseph Lynch component2218819 1 BBa_J70591 component2218817 1 BBa_J70592 annotation2218817 1 BBa_J70592 range2218817 1 1 35 annotation2218819 1 BBa_J70591 range2218819 1 44 55 BBa_J70591_sequence 1 aaagaggagaaa BBa_J70592_sequence 1 ttgacacgctggtaggagcaacttatattctaagc BBa_J70608_sequence 1 ttgacacgctggtaggagcaacttatattctaagctactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z