LOCUS       BBa_J70610              1969 bp    DNA     linear       31-Dec-2025
DEFINITION  assembly of J70607 and S04431
ACCESSION   BBa_J70610
VERSION     BBa_J70610.1  
FEATURES             Location/Qualifiers
     misc_feature    1..186
                     /label=BBa_J70607
     misc_feature    195..198
                     /label=BsaXI partial site (only occurs when follows scar)
     misc_feature    989..999
                     /label=BsaXI site
     misc_feature    1234..1243
                     /label=BsaXI partial site (occurs with suffix or scar)
     misc_feature    700..700
                     /label=mutation from J70568: G506A (DNA), V169I (protein)
     promoter        195..364
                     /label=R0010(-naturally occurring internal BsaXI site)
     RBS             372..384
                     /label=B0034
     CDS             391..1071
                     /label=E1010
     misc_feature    1072..1096
                     /label=Junk from J04450
     misc_feature    206..208
                     /label=BsaXI potential cut site
     misc_feature    977..979
                     /label=BsaXI cut site
     misc_feature    1007..1009
                     /label=BsaXI cut site
     misc_feature    1224..1226
                     /label=BsaXI cut site
     misc_feature    1105..1184
                     /label=B0010
     misc_feature    1193..1233
                     /label=B0012
     misc_feature    195..1243
                     /label=BBa_J70575
     CDS             1250..1969
                     /label=GFP protein
     CDS             1250..1969
                     /label=BBa_E0040
ORIGIN
        1 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
       61 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      121 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag
      181 gagaaatact agagctccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      241 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      301 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      361 cacatactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
      421 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      481 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
      541 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
      601 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
      661 ttcaaatggg aacgtgttat gaacttcgaa gacggtggta ttgttaccgt tacccaggac
      721 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
      781 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
      841 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
      901 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
      961 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1021 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     1081 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1141 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1201 gctcaccttc gggtgggcct ttctgcgttt ataggaggac aggtactaga tgcgtaaagg
     1261 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     1321 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     1381 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     1441 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
     1501 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1561 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     1621 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     1681 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     1741 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     1801 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     1861 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     1921 aacagctgct gggattacac atggcatgga tgaactatac aaataataa
//