BBa_J70630 1 BBa_J70630 RBS-MBP-GFP 2010-08-20T11:00:00Z 2015-05-08T01:08:25Z Maltose binding protein was amplified from the NEB vector pMAL-p5x using the primers RBS-MBP-GS-GFP start f 5'- GAA TTC GCG GCC GCT TCT AGA GAC GAA CGC TCT CTA CTA GAT GCC TAG AGT CG C CCC CTA AGG GCG GAG GTA GGA GAA ACT CAA ATG AAA ATC GAA GAA GGT AAA CTG GTA ATC TG -3' RBS-MBP-GS-GFP start r 5'- CTG CAG CGG CCG CTA CTA GTA GTA ATA TAT GTT CGA TAG ATT TTA CGA GAA CC A GTC TGC GCG TCT TTC AGG -3' RBS and maltose binding protein sequence is inserted into part J70423 to create R0010-J70630. This is the sequence that results on the right side of the part. false false _41_ 0 1201 41 Not in stock false the enzyme psr is used to both cut J70423 to insert the rbs mbp part and to cut the amplified rbs mbp part false Julie Norville annotation2078462 1 maltose binding protein range2078462 1 36 1136 annotation2078463 1 gly-ser linker range2078463 1 1137 1142 annotation2078461 1 RBS range2078461 1 1 35 annotation2078464 1 E0040-start sequence range2078464 1 1143 1858 BBa_J70630_sequence 1 tcgccccctaagggcggaggtaggagaaactcaaatgaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgccgccagtccgaacaaagagctggcaaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggtgaaagatccgcgtattgccgccactatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactggttctcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z