LOCUS       BBa_J70631              2066 bp    DNA     linear       15-Nov-2024
DEFINITION  R0010-J70630, rbs and maltose binding protein added to J70423
ACCESSION   BBa_J70631
VERSION     BBa_J70631.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..243
                     /label=RBS
     CDS             244..1344
                     /label=maltose binding protein
     misc_feature    1345..1350
                     /label=gly-ser linker
     CDS             1351..2066
                     /label=E0040-start sequence
     CDS             209..2066
                     /label=BBa_J70630
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc gccccctaag ggcggaggta ggagaaactc
      241 aaatgaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
      301 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
      361 atccggataa actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca
      421 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
      481 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
      541 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
      601 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
      661 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
      721 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
      781 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
      841 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
      901 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
      961 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
     1021 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt
     1081 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
     1141 tgggtgccgt agcgctgaag tcttacgagg aagagttggt gaaagatccg cgtattgccg
     1201 ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
     1261 tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
     1321 aagccctgaa agacgcgcag actggttctc gtaaaggaga agaacttttc actggagttg
     1381 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     1441 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     1501 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
     1561 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     1621 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     1681 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     1741 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     1801 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     1861 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     1921 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     1981 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     2041 gcatggatga actatacaaa taataa
//