BBa_J70634 1 BBa_J70634 Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment 2010-11-01T12:00:00Z 2015-05-08T01:08:25Z PCR from the M. florum genome. Primers: * ttccgaattcgcggccgcttctagag tccaaatagtgtggatac,Mfl2I-up-F * ttccactagtaaagacatctatgtttttttac,Mfl2I-up-R Approximately 450 bp upstream DNA fragment of M. florum genome from sites 352951-353409 intended as a recombination site for homologous recombination, removing the restriction enzyme and methylase from M. florum. false false _42_41_48_1_ 0 6 48 Not in stock false Avoidance of biobrick restriction sites, possible primer binding sites. false Tom Knight BBa_J70634_sequence 1 tccaaatagtgtggatactgaactacttttaaatgacttgcaagctttaaaagactatcaaccaatcaaggtgccggtttatgattttaaaacttcttcaagaaccaaagaaacaattacagttaaacctagtgatgttgtaatacttgatggaatttttgcactagctattaaagagattagaaaacttggagatatcaaaatttttataaaaacagctaatgatttaaggtttattagacgtttacaaagagatgtaaatgaaagagggagaactttagaaagtgttgttgaacaatacttaaatgaagttaaaccaatgcatgatgcatttattgggccaagtattgaatacgctgatttaataattccttataaagaggggaatgaagttgcagttgatttagtagcaactaaaatattaacattgttaacagagtaaaaaaacatagatgtctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z